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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 2511-2516
DOI: https://doi.org/10.20546/ijcmas.2020.910.301


Evaluation of Buffalo (Bubalus bubalis) Oocyte Protein Content at Different Stages of Maturation
Raghvendra Kumar* and Shalini Jaswal
1Animal Biotechnology Centre, ICAR-National Dairy Research Institute Karnal, Haryana 132001, India
*Corresponding author
Abstract:

The protein content of buffalo oocyte was estimated using Bradford assay. The sample (oocyte) size was five hundred in a technical replicate. Each group were of different stages of maturation and contained of four technical replicate. These stages were immature zonapellucida free oocyte (IZP-), immature with zonapellucida (IZP+), in vitro mature stage with zonapellucida (IVITMZP+) and in vivo matured with zonapellucida (IVIVZP+). The mean protein content of oocyte groups were93.72±2.76, 107.14±3.79, 110.04±3.46, 116.11±3.77 ng/oocyte respectively for IZP, IZP+, IVITMZP+,IVIVZP+ groups respectively. Protein content was highest in in vivo matured oocytes per oocyte. From this study, it was estimated that approximately 13% of proteins were found in zonapellucida of immature oocyte. Also it was observed, that proteins content in in vivo matured group was higher as compared to in vitro matured group. This variation in protein content is a function of halt and resumption of meiosis during the progression of maturational stages.


Keywords: Oocyte, Protein Content, Oocyte Maturation, in vitro maturation, in vivo maturation, Zona Pellucida
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How to cite this article:

Raghvendra Kumar and Shalini Jaswal. 2020. Evaluation of Buffalo (Bubalus bubalis) Oocyte Protein Content at Different Stages of Maturation.Int.J.Curr.Microbiol.App.Sci. 9(10): 2511-2516. doi: https://doi.org/10.20546/ijcmas.2020.910.301