The protein content of buffalo oocyte was estimated using Bradford assay. The sample (oocyte) size was five hundred in a technical replicate. Each group were of different stages of maturation and contained of four technical replicate. These stages were immature zonapellucida free oocyte (IZP-), immature with zonapellucida (IZP+), in vitro mature stage with zonapellucida (IVITMZP+) and in vivo matured with zonapellucida (IVIVZP+). The mean protein content of oocyte groups were93.72±2.76, 107.14±3.79, 110.04±3.46, 116.11±3.77 ng/oocyte respectively for IZP, IZP+, IVITMZP+,IVIVZP+ groups respectively. Protein content was highest in in vivo matured oocytes per oocyte. From this study, it was estimated that approximately 13% of proteins were found in zonapellucida of immature oocyte. Also it was observed, that proteins content in in vivo matured group was higher as compared to in vitro matured group. This variation in protein content is a function of halt and resumption of meiosis during the progression of maturational stages.

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