Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 1017-1025
DOI: https://doi.org/10.20546/ijcmas.2020.910.122


Biocompatibility Study of Collagen I Modified PCL/Gelatin/Chitosan Composite Nanofibrous Scaffold for Fibroblast and Osteoblast Cells
Sneh Gautam*
Department of Molecular Biology and Genetic Engineering, C.B.S.H, G. B. Pant University of Agriculture & Technology, Pantnagar- 263145, Uttarakhand, India
*Corresponding author
Abstract:

In present study, a previously fabricated tripolymer composite scaffold of PCL/gelatin/chitosan was modified with collagen I and the modified scaffold were studied for biocompatibility of L929 mouse fibroblasts and human osteoblast cells. Phase contrast microscopy confirmed the viability of L929 mouse fibroblasts and human osteoblasts cultured with the modified composite scaffold which indicated that scaffold did not produce any toxic effect on both cells. Giemsa staining demonstrated the fibroblastic characteristic morphology of L929 and osteoblastic characteristic morphology of human osteoblast cells. FE-SEM study illustrated the adhesion and proliferation of L929 fibroblasts and osteoblasts, and retained their characteristic morphology on the modified PCL/gelatin/chitosan composite scaffold. Therefore, these results indicated that both cells were viable, proliferating, and showing their phenotypic morphology on collagen I modified PCL/gelatin/chitosan composite nanofibrouss caffold. Hence, the collagen I modified PCL/gelatin/chitosan composite nanofibrouss caffold might be an acceptable contender for skin and bone tissue engineering applications.


Keywords: Composite scaffold, L929 mouse fibroblasts, Human osteoblasts, Polycaprolactone, Gelatin, Chitosan, Collagen I
Download this article as Download

How to cite this article:

Sneh Gautam. 2020. Biocompatibility Study of Collagen I Modified PCL/Gelatin/Chitosan Composite Nanofibrous Scaffold for Fibroblast and Osteoblast Cells.Int.J.Curr.Microbiol.App.Sci. 9(10): 1017-1025. doi: https://doi.org/10.20546/ijcmas.2020.910.122