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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 683-692
DOI: https://doi.org/10.20546/ijcmas.2020.910.082


In vitro Callus Induction of Green Bamboo
A. S. Mali*, N. S. Chavan and N. R. Chavan 
MGM College of Agricultural Biotechnology (CABT), Vasantrao Naik Marathwada Krishi Vidyapeeth, Gandheli, Aurangabad-431003, Maharashtra, India
*Corresponding author
Abstract:

Bamboo farming is one of the most promising business in India due to high productivity and assured yield under adverse conditions. The increasing demand of bamboo plantlets can be not be met by traditional cultivation methods. Plant tissue culture techniques have potential for producing large number of healthy plantlets for ever-growing demand. Hence for in vitro culture techniques induction of callus is necessary which can be further used for organogenesis and somatic embryogenesis. The present study demonstrates a simple and efficient method for callus induction of Bamboo. The leaf, node and seed are used as explants for induction of callus. These explants were subjected to sterilization treatment and it is found that Tween -20, bavistin and ethanol are sufficient for proper sterilization of explants. Then these explants were subjected to various concentrations of growth hormone 2,4-Dichlorophenoxyacetic acid (2,4-D) added in Murashige and Skoog Media. It was concluded that the seeds are suitable explants for rapid induction of callus. Then the various concentration of 0 to 8 mg/lit of 2,4-Dichlorophenoxyacetic acid was used and it was observed that 5 mg/lit concentration is best suited for growth of callus under in vitro condition when all other parameters are kept constant.


Keywords: Bamboo, Plant tissue Culture, Callus, 2,4-Dichlorophenoxyacetic acid.
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How to cite this article:

Mali, A. S., N. S. Chavan and Chavan, N. R. 2020. In vitro Callus Induction of Green Bamboo.Int.J.Curr.Microbiol.App.Sci. 9(10): 683-692. doi: https://doi.org/10.20546/ijcmas.2020.910.082