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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 310-322
DOI: https://doi.org/10.20546/ijcmas.2020.910.039


Deepthi Gowda, S., S. Amruta Bhat, V. Kantharaju, Sandhyarani Nishani, Abdul Kareem and Hadimani, H. P. 2020. Pathogenic Variability and Fungicidal Sensitivity of Fusarium spp. Causing Wilt in Chilli in Karnataka.
Usha Kiran1*, Manmohan Sharma1*, Punya1, R. K. Salgotra1, Bikram Singh2 and Amrinder Singh1
1School of Biotechnology,
2Division of Plant Breeding and Genetics, SKUAST-J, Chatha, Jammu (J&K), India
*Corresponding author
Abstract:

A major issue in rice production is the control of Magnaporthe oryzae, the causal agent of rice blast. K 343 being a well adapted variety in the hill zone of Jammu and Kashmir region is susceptible to blast. Present study was undertaken to introgress the broad spectrum resistance gene Pi9 through marker assisted backcross breeding in order to avoid the losses due to this fungus. Foreground selection Pi9 gene in BC2F1 (K 343*3/RML 22) population with marker AP5930 identified 30 target gene positive plants. Background selection for analysis of recurrent parent genome in target gene positive plant using genome wide polymorphic SSR markers led to identification of three plants three plants (P3, P11, P28) in BC2F1 which had recurrent parent genome recovery more than 85 percent. These plants exhibited broader similarity with recurrent parent with respect to agro-morphological traits and resistant to highly resistant reaction to PLP-1 strain of rice blast fungus M. oryzae. It indicates the effectiveness of resistance provided Pi9 gene. These plants would serve as genetic stocks for development of blast resistant lines/varieties or donor for development of blast resistant varieties.


Keywords: Rice, Blast disease, Pi9 gene, Foreground selection, Background selection
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How to cite this article:

Usha Kiran, Manmohan Sharma, Punya, R. K. Salgotra, Bikram Singh and Amrinder Singh. 2020. Development and Characterization of Blast Resistant Genetic Stocks in Rice for Pi 9 Gene through Backcross Breeding.Int.J.Curr.Microbiol.App.Sci. 9(10): 310-322. doi: https://doi.org/10.20546/ijcmas.2020.910.039