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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 281-291
DOI: https://doi.org/10.20546/ijcmas.2020.910.036


Isolation and Purification of Bacteriocin Produced by Lactobacillus plantarum Isolated from Honey Bees Gut and its Inhibitory action against Major Human Colon Pathogens
Swapnil S. Khetre*, K G. Deshpande and Prathamesh N. Bakshi
MGM College of Agricultural Biotechnology (CABT), Vasantrao Naik Marathwada Krishi Vidyapeeth Gandheli, Aurangabad- 431003, Maharashtra, India
*Corresponding author
Abstract:

Lactobacillus plantarum plays an important role in probiotics as Lactobacillus species have been extensively used since decades against infectious diseases and is widely studied for their ability to protect against pathogens. This Lactobacillus plantarum is used regularly on various probiotics based food to maintain good digestion and pH of fluids secreted in colon of humans for healthy digestion. The present work aims to critically evaluate some central methods and procedure to know behaviour of L.plantarum at various ranges of pH and temperature. For this Honey bee was used for isolation of Lactobacillus plantarum. This L.plantarum was isolated from colon part of honey bee especially from rectum region of stomach. The bacteriocin isolated from Lactobacillus plantarum obtain from gut of honey bee was also used to study inhibitory effect against major human colon pathogens (shigella, E.coli, Staph.aureus and Salmonella typhimurium) and its inhibition was studied and result obtain on various basis was noted. As majority of pathogens shows inhibition against bacteriocin produced these plays a key role in treatment of various diseases caused by pathogenic bacteria and has serious effects on human’s health.


Keywords: Bacteriocin, Lactobacillus plantarum, Honey bee, Antimicrobial activity
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How to cite this article:

Swapnil S. Khetre, K G. Deshpande and Prathamesh N. Bakshi. 2020. Isolation and Purification of Bacteriocin Produced by Lactobacillus plantarum Isolated from Honey Bees Gut and its Inhibitory Action against Major Human Colon Pathogens.Int.J.Curr.Microbiol.App.Sci. 9(10): 281-291. doi: https://doi.org/10.20546/ijcmas.2020.910.036