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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 2858-2866
DOI: https://doi.org/10.20546/ijcmas.2020.909.353


Isolation of RNA from Grains of Medicinal Rice “Njavara” using Improved TRIzol Method
S. Wagh, M. M. Viji, K. B. Soni, P. R. Jadhav, S. C. Ekatpure, V. Manju, Roy Stephen and R. Beena
College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, Kerala, India – 695522
*Corresponding author
Abstract:

Isolation of high-quality RNA from seeds is very critical for seed specific gene analysis. An investigation and comparison on the use of CTAB, SDS and TRIzol extraction procedures to extract high-quality RNA from grains of medical njavara rice were carried out in the present study. These protocols either failed to yield RNA or resulted in reduced yield with poor quality of RNA from Njavara rice grains. The starch and secondary metabolites present in njavara rice seeds hindered the isolation and the resuspension of precipitated RNA or contaminated the RNA pellets by co-precipitation. Hence, we modified the TRIzol RNA extraction protocol by addition of 0.5% N-lauryl sarcosine, 2% β-Mercaptoethanol and 1% PVP.  Highly pure (A260/A280 ratio ranged from 1.9 to 2.0) and intact RNA with a higher yield (up to 500µg/ml of RNA) could be obtained using modified TRIzol RNA extraction protocol. RNA obtained was testified and efficiently utilized for the cDNA preparation and amplification of Ubiquitin gene.


Keywords: Medicinal rice, Njavara rice, Seeds, RNA Isolation, TRIzol, SDS, CTAB
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How to cite this article:

Wagh, Y. S., M. M. Viji, K. B. Soni, P. R. Jadhav, S. C. Ekatpure, R. V. Manju, Roy Stephen and Beena, R. 2020. Isolation of RNA from Grains of Medicinal Rice “Njavara” using Improved TRIzol Method.Int.J.Curr.Microbiol.App.Sci. 9(9): 2858-2866. doi: https://doi.org/10.20546/ijcmas.2020.909.353