National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study, attempts for the isolation of Mycoplasma organisms were carried out in PPLO broth and PPLO agar medium. Out of 253 sheep and 112 goat nasal swab samples inoculated in PPLO broth, 27 sheep and 14 goat samples showed colour change in broth. Upon sub-culturing of 27 sheep and 14 goat broth nasal swab samples on PPLO agar, 17 sheep and 9 goat samples produced colonies. Positive samples produced yellow color change to broth medium with mild turbidity after 48 hrs of incubation at 37°C in CO2 incubator maintaining 5% CO2. After 7th day of incubation on PPLO agar, the agar plates showed fried egg colonies and granular colonies when examined under 10X magnification of microscope. Further Mycoplasma isolation was confirmed by PCR targeting 16S rRNA gene of genus Mycoplasma and Mycoplasma ovipneumoniae.
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