National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objective of this study was to enhance the production of α-amylase enzyme in some Bacillus strains through mutation techniques because of the exponential increase in demand for their usage in various fields. Seven Bacillus strains; B. licheniformis, B. alvei, B. thuringensis, B. subtilis, B. pumilus, B. circulants, and B. cereus were used in this study. The highest productive strains were B. licheniformis and B. subtilis. They were treated with UV irradiation and Ethyl methanesulfonate (EMS) as physical and chemical mutagens, respectively. Results revealed that the survival rate using either UV or EMS treatment was decreased gradually with the increase of exposure time. In case of UV mutagenesis, out of one hundred and fifty mutants, thirty-three were highly productive than parental strains. The highest two mutants resulted from B. subtilis were, BSUV5 and BSUV2 after 15 min. of exposure, i.e., 42.71 and 39.57 U/ml, respectively. They gave productivity thirty-three-fold and thirty-one-fold than parental strain. On the other hand, the highest three mutants resulted from B. licheniformis were, BLUV14, BLUV11(60 min. of exposure) and BLUV3 (15 min. of exposure), i.e., 42.86, 41.57 and 40.00 U/ml, respectively. The highest BLUV14 mutant gave productivity of thirty-seven-fold than parental strain. On the other hand, in case of EMS mutagenesis, out of twenty-two mutants resulted from B. licheniformis which was chosen to be treated with EMS, five potent mutants were higher in enzyme productivity. The highest two mutants were, BLEMS1 (at 250 µl /10 min.) and BLEMS5 (at 1500 µl /60 min.), i.e., 42.93 and 30.67 U/ml, respectively. Plasmid curing of parental strain by elevated temperature or EtBr has not any impact on enzyme production. Finally, these results suggest that induction of mutation was effective in getting super strains in productivity compared to what can be obtained by parental strains.
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