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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 1266-1277
DOI: https://doi.org/10.20546/ijcmas.2020.909.155


Mutagenic Improvement of Some Bacillus Strains for Enhanced Alpha Amylase Production
Effat A. M. Soliman1*, Nariman A.H. Aly1, Nivien A. Abosereh1,Mohammed H.Z. Mutawakil2 and Abdullah S. M. Alotaibi2
1Microbial Genetic Dept., National Research Centre, 33 El-Bohouth St.
(Former El-Tahrir St.) Dokki, Giza, Egypt, P.O. 12662
2Biological Science Dept., Fac. of Science, King Abdulaziz University, Jeddah, Saudi Arabia
*Corresponding author
Abstract:

The objective of this study was to enhance the production of α-amylase enzyme in some Bacillus strains through mutation techniques because of the exponential increase in demand for their usage in various fields. Seven Bacillus strains; B. licheniformis, B. alvei, B. thuringensis, B. subtilis, B. pumilus, B. circulants, and B. cereus were used in this study. The highest productive strains were B. licheniformis and B. subtilis. They were treated with UV irradiation and Ethyl methanesulfonate (EMS) as physical and chemical mutagens, respectively. Results revealed that the survival rate using either UV or EMS treatment was decreased gradually with the increase of exposure time. In case of UV mutagenesis, out of one hundred and fifty mutants, thirty-three were highly productive than parental strains. The highest two mutants resulted from B. subtilis were, BSUV5 and BSUV2 after 15 min. of exposure, i.e., 42.71 and 39.57 U/ml, respectively. They gave productivity thirty-three-fold and thirty-one-fold than parental strain. On the other hand, the highest three mutants resulted from B. licheniformis were, BLUV14, BLUV11(60 min. of exposure) and BLUV3 (15 min. of exposure), i.e., 42.86, 41.57 and 40.00 U/ml, respectively. The highest BLUV14 mutant gave productivity of thirty-seven-fold than parental strain. On the other hand, in case of EMS mutagenesis, out of twenty-two mutants resulted from B. licheniformis which was chosen to be treated with EMS, five potent mutants were higher in enzyme productivity. The highest two mutants were, BLEMS1 (at 250 µl /10 min.) and BLEMS5 (at 1500 µl /60 min.), i.e., 42.93 and 30.67 U/ml, respectively. Plasmid curing of parental strain by elevated temperature or EtBr has not any impact on enzyme production. Finally, these results suggest that induction of mutation was effective in getting super strains in productivity compared to what can be obtained by parental strains.


Keywords: Bacillus, UV irradiation, Ethyl methanesulfonate (EMS), α-amylase, Genetic improvement
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How to cite this article:

Effat A. M. Soliman, Nariman A.H. Aly, Nivien A. Abosereh, Mohammed H.Z. Mutawakil and Abdullah S. M. Alotaibi. 2020. Mutagenic Improvement of Some Bacillus Strains for Enhanced Alpha Amylase Production.Int.J.Curr.Microbiol.App.Sci. 9(9): 1266-1277. doi: https://doi.org/10.20546/ijcmas.2020.909.155