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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 222-231
DOI: https://doi.org/10.20546/ijcmas.2017.604.026


Clinico-Seroepidemiology and Molecular Characterization of Brucellosis in Animals
Kirit B. Patel*, H.C. Chauhan, B.K. Patel, S.S. Patel, M.D. Shrimali, J.K. Kala, A.N. Modi, Rajgor Manish, M.A. Patel, A.C. Patel, M.G. Patel and B.S. Chandel
Department of Animal Biotechnology and Microbiology, College of Veterinary Science and Animal Husbandry Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar- 385 506, Gujarat, India
*Corresponding author
Abstract:

The present study reports clinico seroepidemiological observations of Brucellosis including molecular epidemiology. During the period of study a total of 6410 sera were collected, which included cattle (2723), buffaloes (894), sheep (1072), goats (1281), camel (438) and equine(02). These sera were screened for the presence of Brucella antibodies by RBPT and i-ELISA. The overall seroprevalence detected in animals were 12.85% (824 out of 6410) and 11.87% (761 out of 6410) by RBPT and i-ELISA, respectively. Clinical conditions wise seroprevalence was recorded in heifer 4.65% and 6.97%, clinically healthy animals 7.23% and 6.56%, animals with the history of abortion 25.58% and 29.09%, hygroma 13.33% and 11.66%, pregnant 5.45% and 4.21%, non-pregnant 7.81% and 6.17%, status unknown 7.74% and 6.16%, still birth 16.00% and 20.00%, retention of placenta 14.22% and 11.71%, repeat breeding 19.70% and 14.35%, orchitis 34.34% and 18.18%respectively by RBPT and i-ELISA. Out of 744 milk samples of aborted animal, 152 milk samples found positive for Brucella antibody by MRT. A total l110 clinical samples were processed by genus specific PCR using B4/B5 (223bp) primer. Of these 15 samples positive for Brucella organism. Out of 15 genus specific PCR positive samples, 12samples positive by B. abortus +IS711 (498bp) species specific PCR and 3 samples positive by B. melitensis omp31 (723bp) species specific PCR. All the 15 samples also confirmed positive by Bruce ladder PCR.


Keywords: Brucellosis, Seroprevalance, PCR, B. abortus,melitensis, Molecular detection.
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How to cite this article:

Kirit B. Patel, H.C. Chauhan, B.K. Patel, S.S. Patel, M.D. Shrimali, J.K. Kala, A.N. Modi, Rajgor Manish, M.A. Patel, A.C. Patel, M.G. Patel and Chandel, B.S. 2017. Clinico-Seroepidemiology and Molecular Characterization of Brucellosis in Animals.Int.J.Curr.Microbiol.App.Sci. 6(4): 222-231. doi: https://doi.org/10.20546/ijcmas.2017.604.026