National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Snake bite is a major public health concern which can cause bacterial infection along with envenomation. The infection may be due to the bacteria present in mouth of venomous or nonvenomous snake which may contaminate the bite site during biting. In the present study coagulase negative Staphylococcus species from the oral cavity of two Indian cobras were isolated and identified and were screened for its biofilm forming ability using bentonite clay as a substrate. Various methods like Congored agar assay and tissue culture plate method were also used for quantitative estimation of biofilm formation. Oral swabs were collected from a 5year old female and 8year old male Indian cobra in an aseptic manner after observing the guidelines of capturing the wild snakes by professional environmentalists. The swab samples were processed as per routine microbial procedure and it was found that mostly coagulase negative Staphylococcus species got isolated from both the samples. Pure culture of Staphylococcus species were screened for various biochemical tests like coagulase test, catalase test etc. Gram positive, coagulase negative, catalase positive and growth in mannitol salt agar reveals the presence of characteristic Staphylococcus species and its detailed antimicrobial profile was conducted for public health point of view.
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