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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 232-242
DOI: https://doi.org/10.20546/ijcmas.2020.909.029


Isolation and Characterization of a Novel RIP from Trichosanthes dioica having Anticancer Activity
Tapu Ghosh1*, Laishram Indira Singha2 and Keshab Barman3
1Dept. of Biotechnology, Assam Don Bosco University, Sonapur, Assam, India
2Dept. of Biotechnology, St. Anthony’s College, Shillong, Meghalaya, India
3ICAR-NRC on Pig, Rani, Guwahati, Assam India
*Corresponding author
Abstract:

Plants of Cucurbitaceae family have shown to possess proteins like ribosome-inactivating proteins (RIPs) which have shown promising results against microbes and are also being explored for anti-cancer activities. The present study was designed to isolate a novel protein from a Cucucrbitaceae species which shows Ribosome inactivating property, and which in turn may be effective against cancer i.e. showing anticancer activity. Trichosanthes dioica seeds were taken which are not explored enough for proteins which can inactivate ribosomes and in turn may be used for their medicinal property in cancer treatment etc. Purification for Ribosome Inactivating Proteins (RIPs) resulted in an approx. 32KDa band from Trichosanthes dioica seeds. Ribosome inactivating property was tested using rabbit reticulocyte which gave a positive result.  Anticancer activity of the isolated protein was tested on cancerous cell lines MDA and HL-60 along with PBLs as control cell line. The results showed that the protein is effective against cancerous cell lines with minimal effect on normal cell lines. The isolated protein was found to be less toxic to normal cells and more effective as anti-proliferative agents than Momordica charantia derived protein which was used as positive control. Also, the isolated protein showed a protein profile with predominance of alpha-helical elements. The study showed that N-glycosidase activity of the protein plays a great role as the mode of action as found in the secondary structure homology analysis.


Keywords: Anticancer, Cucurbitacea, MTT-Assay, Purification, Ribosome inactivating property, Secondary structure
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How to cite this article:

Tapu Ghosh, Laishram Indira Singha and Keshab Barman. 2020. Isolation and Characterization of a Novel RIP from Trichosanthes dioica having Anticancer ActivityInt.J.Curr.Microbiol.App.Sci. 9(9): 232-242. doi: https://doi.org/10.20546/ijcmas.2020.909.029