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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 79-87
DOI: https://doi.org/10.20546/ijcmas.2017.604.010


Diversity Analysis among Fusarium oxysporum F. Sp. cumini Isolates Using ISSR Markers, Spore Morphology and Pathogenicity
Bhagwat Nawade1*, J.R. Talaviya, U.M. Vyas2, K.B. Jadeja2 and B.A. Golakiya1
1Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, 362001 Junagadh, Gujarat, India
2Department of Plant Pathology, College of Agriculture, Junagadh Agricultural University, 362001 Junagadh, Gujarat, Indiaa
*Corresponding author
Abstract:

Fusarium oxysporum f. sp. cumini is a serious disease of cumin (Cuminum cyminum L.). Ten isolates of F. oxysporum f. sp. cumini were isolated from major cumin growing districts of Gujarat state to compare molecular methods over traditional for differentiation among the isolates of pathogen. The average 98.70 % of polymorphism was recorded for all the 11 ISSR primers. The polymorphic information content (PIC) value ranged from 0.571 to 0.831 with an average of 0.739 per primer. The IISR primers grouped majority of the isolates based on their geographical location. The microconidia and macroconidia were 0-1 and 2-5 septa respectively and hyaline. All isolates showed varied levels of virulence. The ISSR analysis grouping does not show correlation with spore morphology and pathogenicity of the isolates.


Keywords: Cumin wilt, Genetic diversity, Inter Simple Sequence Repeat, Virulence
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How to cite this article:

Bhagwat Nawade, J. R. Talaviya, U.M. Vyas, K.B. Jadeja and Golakiya, B.A. 2017. Diversity Analysis among Fusarium oxysporum F. Sp. cumini Isolates Using ISSR Markers, Spore Morphology and PathogenicityInt.J.Curr.Microbiol.App.Sci. 6(4): 79-87. doi: https://doi.org/10.20546/ijcmas.2017.604.010