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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3092-3097
DOI: https://doi.org/10.20546/ijcmas.2020.908.350


Studies on the Effect of Growth Regulators on In-vitro cloning of Carica papaya L. through Shoot Tip and Inflorescences
Anuj Pal*, Yogesh Prasad Rajbhar, Harvindra Pal and Govind Rajbhar
Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut 250110. (U.P.) India
*Corresponding author
Abstract:

Papaya is the most important fruit crop of tropical and sub-tropical countries of the world. It is valued for its nutritional as well as pharmaceutical importance as it is a rich source of provitamin A, Calcium and Papaina photolytic enzyme. Since Papaya is highly heterozygous fruit crop. There is a need for multiplication of plant through Tissue Culture for bearing fruits in almost all the plants. Keeping these facts in view the multiplication of Papaya Carica papaya L. cv. Pusa Delicious through Tissue Culture is needed for the production of numerous plants within a short period that is why the present experiment was carried out in the Tissue Culture Lab of the Department of Horticulture, Sardar Vallabhbhai University of Agriculture & Technology, Meerut, (U.P.) during the year 2016-2017. The experiment was conducted in two way factorial design with 33 treatments comprising of 3 level of BAP (1,2 and 3 ppm), 3 levels of Kinetin(1, 1.5 and 2 ppm), 6 level of NAA(0.01, 0.1, 0.5, 1, 2 and 3 ppm) and 5 levels of IBA(0.1, 0.5, 1, 2 and 3 ppm) replicated thrice. The different concentrations of Auxins and Cytokinins alone and in various combinations are applied. The effect of these combinations was observed among the different concentration of the bio-regulators, BAP 1 ppm + NAA 0.5 ppm was found to be the best for shoot development and as well as for maximum number of shoot. In case of rooting of explants, the effect of IBA and NAA was studied with the half dose of CHU (N6) media, NAA 3 ppm was most effective in terms of root induction in explant which induced the root just in 11.66 days. IBA 1 ppm was best in inducing maximum number of roots. Hence based on result obtained from the investigation it can be concluded that the combination of BAP 1 ppm and NAA 0.5 ppm is the best for shoot development and NAA 3 ppm and IBA 1 ppm is the best for early rooting and maximum rooting respectively in CHU (N6) media for Papaya (Carica papaya L.) tissue culture.


Keywords: Carica papaya, Auxins and Cytokinins
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How to cite this article:

Anuj Pal, Yogesh Prasad Rajbhar, Harvindra Pal and Govind Rajbhar. 2020. Studies on the Effect of Growth Regulators on In-vitro Cloning of Carica Papaya L. through Shoot Tip and Inflorescences.Int.J.Curr.Microbiol.App.Sci. 9(8): 3092-3097. doi: https://doi.org/10.20546/ijcmas.2020.908.350