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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 2856-2869
DOI: https://doi.org/10.20546/ijcmas.2020.908.321


Role of Catalase as Semen Additive in Cryopreservation of Cattle Bull Semen
Padamveer Singh1, Sanjay Agarwal1*, Harjyote Singh1, Ajay Kumar Sharma4, Pawan Kumar Vermaand Sharad Kumar3
1Divison of Veterinary Gynaecology & Obstetrics, 2Division of Veterinary Pharmacology and Toxicology, 3Division of Teaching Veterinary Clinical Complex, F.V.Sc. & A.H., S.K.U.A.S.T.-J., R. S. Pura-181102, Jammu, India 4Frozen Semen Laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu, India
*Corresponding author
Abstract:

The present study was done at the Frozen Semen Laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu and Division of V.G.O., F.V.Sc & A.H., SKUAST- J, R.S.Pura, during the period between December 2017 and May 2018. This investigation was carried out with the objective to study the effect of antioxidant semen additive catalase on cryopreservation of semen. Semen samples (n=10) from mature cattle bull stationed at Frozen semen laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu, were used to evaluate the effect of catalase additive at post-dilution and at post thaw stage. The semen sample was extended with Tris-Egg-Yolk-Citric-acid-Fructose-Glycerol (TEYCAFG) extender and were split into two groups: Group 1: TEYCAFG without any additive/ control and group 2: TEYCAFG + Catalase (100 IU/ml). Progressive motility, live spermatozoa, acrosomal integrity, sperm abnormality, hypo-osmotic swelling test (HOST) was evaluated at both post-dilution and post-thaw stage. Whereas, oxidative stress tests viz. malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) were evaluated at only post-thawed stage. Group 2 i.e. catalase group, showed significant (p<0.05) increased progressive motility, live spermatozoa, acrosomal integrity and HOST positive spermatozoa, while significant (p<0.05) decreased sperm abnormalities in post-thawed semen. In oxidative stress evaluation the MDA level was significantly (p<0.05) decreased, whereas, CAT and SOD levels significantly (p<0.05) increased in group 2 in comparison to control group. It was concluded that addition of catalase (100 IU/ml) as semen additive improves semen quality and minimize oxidative stress to the spermatozoa during cryopreservation of semen.


Keywords: Semen, Cattle bull, Catalase, Oxidative stress
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How to cite this article:

Padamveer Singh, Sanjay Agarwal, Harjyote Singh, Ajay Kumar Sharma, Pawan Kumar Verma and Sharad Kumar. 2020. Role of Catalase as Semen Additive in Cryopreservation of Cattle Bull Semen.Int.J.Curr.Microbiol.App.Sci. 9(8): 2856-2869. doi: https://doi.org/10.20546/ijcmas.2020.908.321