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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1245-1252
DOI: https://doi.org/10.20546/ijcmas.2020.908.140


Standardization of Cytokinins (BAP and Kinetin) Concentrations  and their Combination with NAA on Regeneration through Seeds in Lime (Citrus aurantifolia)
Khalid Akhundzada, P. Venkatesha Murthy,Venugopala Reddy* and B. N. Sathyanarayana
Department of Horticulture, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka-560065, India
*Corresponding author
Abstract:

In vitro regeneration through seeds were studied in lime (Citrus aurantifolia) by culturing lime seeds on MS media supplemented with BAP (1, 3 and 5 mg/l), Kinetin (1, 3 and 5 mg/l) and their combination with NAA (0.1, 0.5 and 1 mg/l). In reference to increased germination percentage, mean shoot length, mean number of leaves per explant and mean root length, among BAP and Kinetin concentrations, BAP 3 mg/l was found to be the best treatment. In terms of cytokinins and NAA combine effect, BAP 5 mg/l + NAA 0.5 mg/l and Kinetin 3 mg/l + NAA at 0.1 mg/l were found to be the best combinations. Survival was 100 per cent when seeds were used as explant material.


Keywords: BAP, Kinetin, NAA, Lime seed, Germination
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How to cite this article:

Khalid Akhundzada, P. Venkatesha Murthy, M. Venugopala Reddy and Sathyanarayana, B. N. 2020. Standardization of Cytokinins (BAP and Kinetin) Concentrations and their Combination with NAA on Regeneration through Seeds in Lime (Citrus aurantifolia).Int.J.Curr.Microbiol.App.Sci. 9(8): 1245-1252. doi: https://doi.org/10.20546/ijcmas.2020.908.140