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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2241-2251
DOI: https://doi.org/10.20546/ijcmas.2020.907.261


Assessment of Functional and Genetic Diversity of Bacterial Isolates from Uttarakhand Acid Soil
Kiran K. Reddy1, 2*, K. Annapurna1, Dolamani Amat1, 3 and L. Vithal Kumar1
1Division of Microbiology, Indian Agricultural Research Institute, New Delhi-110 012, India
2ICAR-Directorate of Groundnut Research, Junagadh, Gujarat-362 001, India
3ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh-462 038, India
*Corresponding author
Abstract:

In this study a total of 34 bacterial cultures were isolated from Uttarakhand (Rishikesh) acidic soil (pH 5.6) were screened for plant growth promoting traits. Isolations were carried out on nutrient and kings B media in which colony forming unit (CFU) count was log10 6.94 and log10 5.04 respectively. About 17% and 26% of the isolates were found to be antagonistic to plant parasitic fungi Fusarium verticilloides and Rhizoctonia bataticola. Indole acetic acid (IAA) production, P-solubilization potential, siderophore and HCN production was found in 3%, 26%, 12% and 6% of isolates respectively. Genetic diversity was analyzed through 16S rDNA sequencing of six selected isolates showing at least two plant growth promoting (PGP) traits. An abundance Gram-positive Firmicutes like Bacillus spp. (50%) was observed followed by one each strain belonging to α-, β- and γ – proteobacteria. Ochrobactrum spp. LK-8 exhibited different PGP attributes like Siderophore, HCN production and antifungal activity. Bacillus megaterium LK-23 also showed P-solubilization ability and antifungal activity. Phylogenetic tree illustrated two major clusters each comprising of three strains. In brief, bacterial functional and genetic diversity was affected by site location, climate and edaphic factors.


Keywords: Plant growth promotion, Bacillus spp., Ochrobactrum spp., Antifungal, HCN and IAA
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How to cite this article:

Kiran K. Reddy, K. Annapurna, Dolamani Amat and Vithal Kumar, L. 2020. Assessment of Functional and Genetic Diversity of Bacterial Isolates from Uttarakhand Acid Soil.Int.J.Curr.Microbiol.App.Sci. 9(7): 2241-2251. doi: https://doi.org/10.20546/ijcmas.2020.907.261