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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2095-2101
DOI: https://doi.org/10.20546/ijcmas.2020.907.243


Isolation, Identification and Detection of Staphylococcus aureus in Raw Chicken and Frozen Chicken Meat Products in Ludhiana, India by Standard Isolation Techniques and PCR Assay
Nishchal Dutta1*, H.S. Banga2, Sidhartha Deshmukh2, Geeta Devi Leishangthem2 and Nittin Dev Singh2
1Department of Veterinary Pathology, Khalsa College of Veterinary and Animal Sciences, Amritsar-143002, Punjab, India
2Department of Veterinary Pathology, College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
*Corresponding author
Abstract:

Staphylococcus aureus is one of the major food contaminant with life threatening potential in both humans and animals. This pathogen is one of the indicator organism to monitor hygienic condition(s) during slaughter and processing of the meat samples. The present study was aimed at finding the prevalence of Staphylococcus aureus in raw and frozen chicken meat products purchased from different retail outlets and local butcher shops across the Ludhiana city. In the present study a total of 100 raw chicken meat samples were collected (80 fresh raw samples and 20 frozen chicken meat products). During this study, 100 chicken meat samples were inoculated in mannitol salt agar for the selective isolation of S. aureus and were later characterized by a combination of microscopic and bio-molecular tests. Results of the study revealed that, 31 samples were containing S. aureus, which is 31% of total samples. The isolation techniques and PCR assay were found to be more specific, reliable and expeditive methods to corroborate the presence of Staphylococcus aureus in raw and frozen meat products.


Keywords: Staphylococcus aureus, Retail meat, Frozen meat products and PCR
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How to cite this article:

Nishchal Dutta, H.S. Banga, Sidhartha Deshmukh, Geeta Devi Leishangthem and Nittin Dev Singh. 2020. Isolation, Identification and Detection of Staphylococcus aureus in Raw Chicken and Frozen Chicken Meat Products in Ludhiana, India by Standard Isolation Techniques and PCR Assay.Int.J.Curr.Microbiol.App.Sci. 9(7): 2095-2101. doi: https://doi.org/10.20546/ijcmas.2020.907.243