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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2067-2075
DOI: https://doi.org/10.20546/ijcmas.2017.603.236


Genetic Transformation Protocol for Early Flowering Cryptochrome Gene in Taif Rose Plant
Adel E.EL-Tarras1,2*, Salah El-Asaal2 and Ahmed shahaby1,3
1Biotechnology and Genetic Engineering Unit, Scientific Research Deanship,Taif University, KSA
2Department of Genetics, Faculty of Agriculture, Cairo University, Cairo, Egypt
3Department of Microbiology, Faculty of Agriculture, Cairo University, Cairo, Egypt
*Corresponding author
Abstract:

Taif is one of the oldest cities in the world. It is located in the western part of Saudi Arabia, Taif rose is considered as one of the most important economic products of Taif governorate, KSA. The major use of Damask rose is distillation of petals in order to extract its essential oil and producing rosewater. Due to the heterozygsity, high degree of sterility and difficulty of breeding programs in Roses, therefore, the genetic transfer is the appropriate way to increase productivity and improve quality. In the present study genetic transformation in rose depend on tissue culture and gene transfer programmers, The stem nods of the Taif-Rose genotype were used for the development of micropropagation system., the Arabidopsis natural early flowering time allele cape verde island (Cvi ) of cryptochrome 2 (ArCRY2-Cm) gene was used for rose transformation in an attempt to produce early flowering transgenic rose. The reporter gene, GUS, was used for determining transformation efficiency in this study. GUS was carried on several plasmid constructs which also contained antibiotic resistance (kanamycin or streptomycin. Efficiency of gene transfer was determined by calculating the number of transiently expressing GUS cells for each combination of factors.


Keywords: Biotechnology, rose, cryptochrome, genetic transformation, GUS.
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How to cite this article:

Adel E.EL-Tarras, Salah El-Asaal and Ahmed shahaby. 2017. Genetic Transformation Protocol for Early Flowering Cryptochrome Gene in Taif Rose Plant.Int.J.Curr.Microbiol.App.Sci. 6(3): 2067-2075. doi: https://doi.org/10.20546/ijcmas.2017.603.236