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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2055-2059
DOI: https://doi.org/10.20546/ijcmas.2017.603.234


In vitro Efficacy of Antibacterial Proteins from Haemolymph of Silkworm Breeds against Bacterial Pathogens of Mulberry Silkworm, Bombyx mori L.
S. Manimegalai1, B. Bebitha2 and P.Mohanraj3*

1Department of Agricultural Entomology, Tamil Nadu Agricultural University,Coimbatore-3, India
3Department of Sericulture, Forest College and Research Institute, Mettupalayam, India


*Corresponding author
Abstract:

Antibacterial activity of proteins eluted from immunized haemolymph collected from breeds of mulberry silkworm, Bombyx mori L., viz., Rong daizo, CSR2 and double hybrid were studied against bacterial pathogens. The breeds were immunized with bacterial pathogens, Escherichia  coli (KR strain),  Bacillus thuringiensis and Staphylococcus  aureus . In vitro efficacy of purified antibacterial proteins from breeds of  B.mori against E.coli, S. aureus and B. thuringiensis  revealed that  among the ten fractions of antibacterial proteins eluted from B.mori breeds, ninth fraction@ 200µl  showed higher antibacterial activity in Rong daizo (3.01cm, 3.10 cm and 1.82cm) (2.71 cm, 3.01cm and 1.60 cm), CSR2 (2.98 cm, 3.05 cm and 1.81 cm) and double hybrid (  2.89 cm, 2.98 cm and 1.79 cm )  against E.coli, S. aureus and B. thuringiensis (Table 1,2&3).Lesser inhibitory activity was recorded in seventh fraction @ 200 µl concentration of Rong daizo (2.71 cm, 3.01 and 1.60 cm ) followed by CSR2 (2.68 cm, 2.98cm  and 1.59 cm) and DH (2.69 cm, 2.96 cm and 1.54 cm) respectively. Standard antibiotic streptomycin sulphate @ 200 µl concentration produced the inhibition zone of 7.12 cm, 8.12 cm and 6.32 cm. Purified antibacterial protein fractions showed higher antibacterial activity against gram negative E.coli and gram positive S.aureus and low activity against B.thuringiensis.


Keywords: Bombyx mori, Antibacterial, Proteins, Mulberry, Bacillus thuringiensis.
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How to cite this article:

Manimegalai, S., B. Bebitha and Mohanraj, P. 2017. In vitro Efficacy of Antibacterial Proteins from Haemolymph of Silkworm Breeds against Bacterial Pathogens of Mulberry Silkworm, Bombyx mori L.Int.J.Curr.Microbiol.App.Sci. 6(3): 2055-2059. doi: https://doi.org/10.20546/ijcmas.2017.603.234