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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2038-2046
DOI: https://doi.org/10.20546/ijcmas.2017.603.232


Comparison of Antibiotic Resistance and Lipase Production in Extended Spectrum β-lactamases Producing and Non-producing Isolates of Pseudomonas aeruginosa
Alfia Alim, Aparna*, Antariksh Deep, Priyanka Yadav and Uma Chaudhary
Department of Microbiology, Pt. B D Sharma PGIMS, Rohtak, Haryana, India
*Corresponding author
Abstract:

Pseudomonas aeruginosa is a major cause of nosocomial infections. Recently multidrug resistance and extended-spectrum ß-lactamase (ESBL)-producing P. aeruginosa isolates are emerging worldwide. These isolates are reported to be more virulent than the non-multidrug resistance and non ESBL producing isolates. In order to find a correlation between ESBL production and virulence, we tested one virulence factor involved in pathogenicity- lipase production in ESBL and non ESBL producing isolates. A total of 100 samples were evaluated. ESBL was determined phenotypically by CLSI method while lipase production was determined using egg yolk agar. Forty nine isolates produced ESBL out of which 47 (95%) were positive for lipase while 51 isolates were non-ESBL producing out of which 34 (67%) were positive for lipase (p value < 0.05). Antibiotic resistance was also found more in ESBL producers compared to non-ESBL producers. Our data demonstrate that lipase production was higher in the ESBL producing isolates compared to the non-ESBL producing isolates. Lipase production therefore renders ESBL positive isolates more pathogenic.


Keywords: Antibiotic resistance Lipase, Pseudomonas.
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How to cite this article:

Alfia Alim, Aparna, Antariksh Deep, Priyanka Yadav and Uma Chaudhary. 2017. Comparison of Antibiotic Resistance and Lipase Production in Extended Spectrum β-lactamases Producing and Non-producing Isolates of Pseudomonas aeruginosa.Int.J.Curr.Microbiol.App.Sci. 6(3): 2038-2046. doi: https://doi.org/10.20546/ijcmas.2017.603.232