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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 822-832
DOI: https://doi.org/10.20546/ijcmas.2020.907.095


ISSR Marker Based Genetic Diversity Analysis of 35 Garcinia Accessions (Garcinia gummi-Gutta (L) Roxb)
Anu G. Krishnan*, Anu Cyriac, K. Bini, Sible George Varghese
Regional Agricultural Research Station, Kumarakom, Kottayam, Kerala, India
*Corresponding author
Abstract:

Genetic diversity was assessed for 35 garcinia (Garcinia gummi-gutta (L) Roxb) germplasm maintained at Regional Agricultural Research Station, Kumarakom collected from different geographical locations using ISSR markers. Out of the 34 ISSR primers screened, 18 primers were polymorphic and used for further diversity analysis. The products were detected by electrophoresis and analysed using the program NTSYS-PC. Seventy two loci were polymorphic out of the 99 loci amplified. The average Polymorphic Information Content (PIC) obtained for 18 primers was 0.33 and the average marker index was found to be 1.3. Two major clusters were identified at 64% similarity. The clustering pattern did not follow any geographic distribution pattern. The genetic similarity index ranged from 0.55 to 0.95 indicating a genetic diversity of 45% within the germplasm accessions.


Keywords: Gamboge, Garcinia gummi-gutta, ISSR, Molecular markers, Genetic diversity
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How to cite this article:

Anu G. Krishnan, Anu Cyriac, K. Bini, Sible George Varghese. 2020. ISSR Marker Based Genetic Diversity Analysis of 35 Garcinia Accessions (Garcinia gummi-Gutta (L) Roxb).Int.J.Curr.Microbiol.App.Sci. 9(7): 822-832. doi: https://doi.org/10.20546/ijcmas.2020.907.095