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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 854-860
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.097


Utility of Polymerase Chain Reaction in Diagnosis of Acanthamoebaand Microsporidial Keratitis
Namrata Krishna Bhosale1* , Subhash Chandra Parija1 , Jharna Mandal1 and Shashi Ahuja2
1Department of Microbiology, JIPMER, Puducherry, India
2Department of Ophthalmology, JIPMER, Puducherry, India
*Corresponding author
Abstract:

Evaluation of the diagnostic utility of PCR in comparison to the conventional test. Descriptive study. All patients with suspected microbial keratitis presenting between October 2012 to June 2014 at the Ophthalmology OPD, JIPMER hospital. A total of 50 consecutive non-duplicated cases of keratitis were included in the study period of two years. All the samples were subjected to the conventional test like microscopy using Gram stain and modified trichrome stain, and PCR for Acanthamoeba and Microsporidia. Microscopy, PCR, age and sex distribution of  patients with keratitis, risk factors, clinical presentation of patients. Mean age group of the patients in this study was 48.3 years (range 10-90) and majority of them were females (54%). The predominant symptom with which the patients presented in our study was pain (60%).Corneal trauma with vegetative matter was a major risk factor accounting for 20%. Out of the 50 samples, one (2%) of the specimens was positive for Acanthamoeba and two (4%) were positive for microsporidia by PCR, while, none of the specimens was positive by microscopy for Acanthamoeba and Microsporidia on Modified trichrome stained smears. Hence, this establishes the fact that PCR is superior to microscopy as it is a sensitive cum rapid method for the diagnosis of keratitis due to Acanthamoeba and Microsporidia.


Keywords: Acanthamoeba, Microsporidia, keratitis,PCR
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How to cite this article:

Namrata Krishna Bhosale, Subhash Chandra Parija, Jharna Mandal and Shashi Ahuja. 2016. Utility of Polymerase Chain Reaction in Diagnosis of Acanthamoeba and Microsporidial Keratitis.Int.J.Curr.Microbiol.App.Sci. 5(2): 854-860. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.097