International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:6, June, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 3355-3366

In vitro Study of the Compatibility of Three Fungicides with Biocontrol Agents Trichoderma asperellum and Pseudomonas protegens
Benedicto Martínez Coca1 and Edwin Ronnie Gakegne2
1Centro Nacional de Sanidad Agropecuaria (CENSA), Apartado 10, San José de las Lajas, Mayabeque, CP 32700, Cuba
2Instituto de Investigação Agronômica (IIA), CP 405, Chianga, Huambo, Angola
*Corresponding author

The objective of this work was to establish the compatibility under laboratory conditions, between three fungicides (Cuproflow 37.7 % SC, Yaba M GD 66 and Mancozeb 80 % WP) approved and regularly used to control early blight in Cuba and the biocontrol agents Trichoderma asperellum (strain Ta. 85) and Pseudomonas protegens (Pf-5). The effect of the concentrations of the chemical products, on the mycelial growth, sporulation of T. asperellum and the number of colonies of P. protegens was evaluated by the poisoned culture medium technique. The compatibility was classified according to the T value. The results showed that the three fungicides affected the mycelial growth and the sporulation capacity of the strain of T. asperellum. The colonies of the strain of P. protegens were also affected by the recommended field dose for all tested fungicides. Cuproflow excelled for presenting the smallest affectation in all the performed tests, besides, it was also classified as compatible. The results bring out the probability of incorporating the biological control agents under study and Cuproflow37.7 % SC at low concentration in the integrated management of the Alternaria solani, causing early blight of potato.

Keywords: Trichoderma asperellum, Pseudomonas protegens, Fungicides, Early blight, Alternaria solani

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How to cite this article:

Benedicto Martínez Coca and Edwin Ronnie Gakegne. 2020. In vitro Study of the Compatibility of Three Fungicides with Biocontrol Agents Trichoderma asperellum and Pseudomonas protegens.Int.J.Curr.Microbiol.App.Sci. 9(6): 3355-3366. doi:
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