National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To produce cold active protease, investigation was conducted using bacteria isolated from the core sample of Nella Lake, East Antarctica. Isolates were screened for protease using plate assay method followed by submerged fermentation at cold temperature. Potential isolate was identified as Pseudomonas gessardii strain N-86 by molecular technique. Further, investigations were performed to enhance the protease production capabilities by submerged fermentation technique using strain N-86 with various physiological (temperature and pH) and chemical (Carbon, nitrogen, minerals, amino-acids, and various substrates) parameters. Production optimization revealed the maximum protease production by using skim milk (2% w/v), pH 9.5 and 10% v/v of inoculum size at 15°C. Additional potential inducers for protease production were sucrose (1% w/v), L-Glutamine (0.01% w/v) and MgCl2 (0.1% w/v). Final productions with optimized conditions gave 5.42-fold increase in protease production on 72 h of incubation. The present study successfully produced cold-active protease which may have novel properties to degrade protein based wastes which can contribute in cleaning of environment at cold regions without harming the climate and also can be used in various detergent, textile, food and beverage industries effectively. The present study is the first report on Pseudomonas gessardii isolated from Nella Lake, Antarctica producing cold active protease.
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