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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 3119-3134
DOI: https://doi.org/10.20546/ijcmas.2020.906.374


Extracellular Cold Active Alkaline Proteolytic Enzyme Produced By Pseudomonas gessardii Strain N-86 Isolated from Antarctica
Abhas Kumar Maharana1,2* and Shiv Mohan Singh1,3
1Polar Biology Laboratory, National Centre for Antarctic & Ocean Research, Vasco-da-Gama, Goa-403804, India
2Water Quality Management Division-II, Central Pollution Control Board,Parivesh Bhawan, East Arjun Nagar, Delhi-110032, India
3Department of Botany, Institute of Science, Banaras Hindu University, Varanasi-221005, India
*Corresponding author
Abstract:

To produce cold active protease, investigation was conducted using bacteria isolated from the core sample of Nella Lake, East Antarctica. Isolates were screened for protease using plate assay method followed by submerged fermentation at cold temperature. Potential isolate was identified as Pseudomonas gessardii strain N-86 by molecular technique. Further, investigations were performed to enhance the protease production capabilities by submerged fermentation technique using strain N-86 with various physiological (temperature and pH) and chemical (Carbon, nitrogen, minerals, amino-acids, and various substrates) parameters. Production optimization revealed the maximum protease production by using skim milk (2% w/v), pH 9.5 and 10% v/v of inoculum size at 15°C. Additional potential inducers for protease production were sucrose (1% w/v), L-Glutamine (0.01% w/v) and MgCl2 (0.1% w/v). Final productions with optimized conditions gave 5.42-fold increase in protease production on 72 h of incubation. The present study successfully produced cold-active protease which may have novel properties to degrade protein based wastes which can contribute in cleaning of environment at cold regions without harming the climate and also can be used in various detergent, textile, food and beverage industries effectively. The present study is the first report on Pseudomonas gessardii isolated from Nella Lake, Antarctica producing cold active protease.


Keywords: Cold adapted, East Antarctica, Larsemann Hills, Nella Lake, Protease, Psychrotolerant, Sediment core
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How to cite this article:

Abhas Kumar Maharana and Shiv Mohan Singh. 2020. Extracellular Cold Active Alkaline Proteolytic Enzyme Produced by Pseudomonas gessardii Strain N-86 Isolated from Antarctica.Int.J.Curr.Microbiol.App.Sci. 9(6): 3119-3134. doi: https://doi.org/10.20546/ijcmas.2020.906.374