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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
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Int.J.Curr.Microbiol.App.Sci.2017.6(3): 239-246
DOI: https://doi.org/10.20546/ijcmas.2017.603.026


Genetic Analysis and RAPD Polymorphism in Wheat (Triticum aestivum L.) Genotypes
S.D. Tidke1 and P.S. Ranawade2*
1MGM, Institute of Biosciences and Technology, Aurangabad, India
2Modern College of Agricultural Biotechnology, Pune, India
*Corresponding author
Abstract:

Assessment of genotype diversity was studied using released and in pipeline genotypes of wheat, of these 22 were released and 14 were in pipeline. Genetic diversity among 36 wheat genotypes was studied using Random amplified polymorphic DNA (RAPD) analysis. 21 RAPD primers (RPI primers) were used for screening 36 wheat genotypes from which 2,868 fragments were amplified. It was observed that 63.3% bands were polymorphic and 36.4% were monomorphic. The percent of polymorphic bands in banding pattern was calculated and it was highest in RPI-22 (81.3%) while lowest was recorded in RPI-2 (33.8%) and highest PIC value was observed in RPI-22 and RPI-25 (0.88) while lowest in RPI-7 (0.70). Maximum fragments were produced in RPI-1 (200) and minimum in RPI-7 (82). In banding pattern some unique bands were seen, total 7 unique bands were observed. Genetic relationship between wheat genotypes was determined on the basis of Jackard IJ pair wise similarity coefficient values (Similarity coefficient values ranged from 0.09 to 0.99) and dendrogram was generated by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis using dice's similarity matrix through NT-SYS pc software. From dendrogram 5 solitary and 8 clusters were revealed. From above analysis average coefficient values were revealed and highest (0.99) was observed between WSM-175.1 and WSM-163 genotypes while HD-2781 and NIAW-34 represent lowest average similarity coefficient value (0.09). The maximum similarity percentage i.e. 99% was found between WSM-175.1 and WSM-163 and the minimum similarity i.e. 09% was found between HD-2781 and NIAW-34.


Keywords: Wheat, Genetic diversity, RAPD markers, Polymorphism.
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How to cite this article:

Tidke, S.D. and Ranawade, P.S. 2017. Genetic Analysis and RAPD Polymorphism in Wheat (Triticum aestivum L.) Genotypes. Int.J.Curr.Microbiol.App.Sci. 6(3): 239-246. doi: https://doi.org/10.20546/ijcmas.2017.603.026