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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2924-2936
DOI: https://doi.org/10.20546/ijcmas.2020.906.352


Phenotypic Virulence Characterization of Dichelobacter nodosus from the Cases of Ovine Footrot in Andhra Pradesh and Telangana states of India
S. Vijayalakshmi1*, D. Sreenivasulu2, D. Raniprameela3, N. Vinodkumar4 and A. Karthik5
1LRS, Palamaneru, SVVU, India
2SVVU, Tirupati, India
3SLDL, SVVU, Tirupati, India
4Department of Microbiology, CVSc, Tirupati, India
5SRF, SLDL, Tirupati, India
*Corresponding author
Abstract:

Footrot is a contagious disease affecting the interdigital epidermis and living tissues of digits of sheep and goats. The disease is being reported in Andhra Pradesh (AP) and Telangana states of India regularly causing significant economic loss to the sheep husbandry. The causative agent is the anaerobic, gram negative bacteria, Dichelobacter nodosus. A total of sixteen isolates of D.nodosus have been recovered from footrot positive clinical samples collected from AP and Telangana state and further confirmed by PCR targeting 16Sr RNA gene. The isolates were further processed for virulence characterization by Gelatin gel test and elastase test. Gelatin gel test revealed 56.5% of isolates as virulent, 31.25% of isolates as Intermediate and 12.25% of isolates as benign. Whereas elastase test identified only 18.75% of isolates as virulent, 12.5 % of isolates as intermediate, 12.5% of isolates as benign, the rest 56.25% of the isolates did not exhibit any elastase activity.


Keywords: Interdigital epidermis, Economic loss, RNA gene
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How to cite this article:

Vijayalakshmi, S., D. Sreenivasulu, D. Raniprameela, N. Vinodkumar and Karthik, A. 2020. Phenotypic Virulence Characterization of Dichelobacter nodosus from the Cases of Ovine Footrot in Andhra Pradesh and Telangana states of India.Int.J.Curr.Microbiol.App.Sci. 9(6): 2924-2936. doi: https://doi.org/10.20546/ijcmas.2020.906.352