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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2406-2409
DOI: https://doi.org/10.20546/ijcmas.2020.906.295


The Pathogenicity of Cold and Heat Shocked Pasteurella multocida for Rabbit
Makarim K. A. Teirab1*, El Sanousi, S. M.2 and G. E. Mohamed1
1Department of Microbiology, Faculty of Veterinary Medicine, University of Sudan for Science and Technology, Sudan
2Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum
*Corresponding author
Abstract:

The purpose of this study was to study the pathogenecity of cold and heat- shocked bacteria using Pasteurella multocida and rabbits as a model. Pasteurella multocida is an enigmatic pathogen. The capsule and lipopolysaccharide (LPS) of Pasteurella multocida constitute the major components of the bacterial cell surface; they play key roles in a range of interactions between the bacteria and the hosts they colonize or infect. Pasteurella multocida was inoculated into nutrient broth tubes and incubated at 37°C for 24 hours. The culture was shocked at -15°C for 30 mins and ten serial dilutions were done. The dilutions which did not show visible colonies on the recovery media were centrifuged and inoculated intra peritonealy in four rabbits. The rabbits postmortemed and cultures were made from heart blood by puncturing with a sterile Pasteur pipette. The same test was repeated using  treated culture at 50°C and 40°C for 10 mins such heat-treated cells did not lead to death of  the inoculated rabbits.


Keywords: Pathogenicity, postmortemed, intra peritonealy, hemorrhagic septicaemia
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How to cite this article:

Makarim K. A. Teirab, El Sanousi, S. M and G. E. Mohamed. 2020. The Pathogenicity of Cold and Heat Shocked Pasteurella multocida for RabbitInt.J.Curr.Microbiol.App.Sci. 9(6): 2406-2409. doi: https://doi.org/10.20546/ijcmas.2020.906.295