National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The purpose of this study was to study the pathogenecity of cold and heat- shocked bacteria using Pasteurella multocida and rabbits as a model. Pasteurella multocida is an enigmatic pathogen. The capsule and lipopolysaccharide (LPS) of Pasteurella multocida constitute the major components of the bacterial cell surface; they play key roles in a range of interactions between the bacteria and the hosts they colonize or infect. Pasteurella multocida was inoculated into nutrient broth tubes and incubated at 37°C for 24 hours. The culture was shocked at -15°C for 30 mins and ten serial dilutions were done. The dilutions which did not show visible colonies on the recovery media were centrifuged and inoculated intra peritonealy in four rabbits. The rabbits postmortemed and cultures were made from heart blood by puncturing with a sterile Pasteur pipette. The same test was repeated using treated culture at 50°C and 40°C for 10 mins such heat-treated cells did not lead to death of the inoculated rabbits.
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