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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2553-2560
DOI: https://doi.org/10.20546/ijcmas.2020.906.310


Studies on the Protein Profiles of Heat and Cold shocked Bacteria
Makarim K. A. Teirab1*, Emadeldin Hassan Konozy2, El Sanousi, S. M3, G. E. Mohamed1, Sara Abdel Aziz Abdel Rahim2 and Maha Babiker Dafallah2
1Department of Microbiology, Faculty of Veterinary Medicine, University of Sudan for Science and Technology, Sudan
2Biotechnology Department, Africa City of Technology, Khartoum, Sudan
3Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum

*Corresponding author
Abstract:

The purpose of this study was to detect the changes in the protein profiles for bacteria which were subjected to heat and cold- shock compared to the control. For heat shocked test, all bacteria viz., Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus were inoculated into nutrient broth tubes and incubated at 37°C for 24 hours. Then cultures were treated in a water bath at 100°C for 5 mins, 90°C for 10 mins, 80°C for 15 mins, 70°C for 20 mins and 60°C for 25 min. After that the protein profiles and their concentrations were estimated to detect the differences compared to the control. For cold shocked test the cultures were treated at -32°C, -15°C and 0°C for 30 min. After that protein profiles and concentrations were estimated to detect the differences compared to the control. From chromatogram of Staphylococcus aureus it is evident that apparent changes were noticeable upon raising of temperatures from -32°C to 100°C. The protein bands were focused between 75 kDa to 11 kDa. In case of Staphylococcus epidermis, unlike Staphylococcus aureus, there was no apparent electrophoretic difference in the protein expression from low to high temperature .On the other hand, concerning protein estimation, there was an increase in protein by more than 140% as the temperature rises from -32°C to 100°C. Protein bands were focused between 75kDa to 11 kDa. Although there was an apparent variation in the protein composition of Bacillus cereus with gradual increase of temperature, there were no clear electrophoretic bands discrepancies from - 32°C to 100°C.


Keywords: Gel electrophoresis, Chromatogram, Protein profiles, Estimation, Heat and cold-shock
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How to cite this article:

Makarim K. A. Teirab, Emadeldin Hassan Konozy, El Sanousi, S. M, G. E. Mohamed, Sara Abdel Aziz Abdel Rahim and Maha Babiker Dafallah. 2020. Studies on the Protein Profiles of Heat and Cold shocked Bacteria.Int.J.Curr.Microbiol.App.Sci. 9(6): 2553-2560. doi: https://doi.org/10.20546/ijcmas.2020.906.310