National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The purpose of this study was to detect the changes in the protein profiles for bacteria which were subjected to heat and cold- shock compared to the control. For heat shocked test, all bacteria viz., Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus were inoculated into nutrient broth tubes and incubated at 37°C for 24 hours. Then cultures were treated in a water bath at 100°C for 5 mins, 90°C for 10 mins, 80°C for 15 mins, 70°C for 20 mins and 60°C for 25 min. After that the protein profiles and their concentrations were estimated to detect the differences compared to the control. For cold shocked test the cultures were treated at -32°C, -15°C and 0°C for 30 min. After that protein profiles and concentrations were estimated to detect the differences compared to the control. From chromatogram of Staphylococcus aureus it is evident that apparent changes were noticeable upon raising of temperatures from -32°C to 100°C. The protein bands were focused between 75 kDa to 11 kDa. In case of Staphylococcus epidermis, unlike Staphylococcus aureus, there was no apparent electrophoretic difference in the protein expression from low to high temperature .On the other hand, concerning protein estimation, there was an increase in protein by more than 140% as the temperature rises from -32°C to 100°C. Protein bands were focused between 75kDa to 11 kDa. Although there was an apparent variation in the protein composition of Bacillus cereus with gradual increase of temperature, there were no clear electrophoretic bands discrepancies from - 32°C to 100°C.
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