National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Phylloplane and rhizosphere microorganism were isolated from tropical sugarbeet. Phyllolplane fungi viz., Cladosporum sp., Penicillium sp., Aspergillus niger, Aspergillus flavus, and Curvularia and Bacteria of B. subtilis and P. fluorescens were isolated seven phylloplane microorganisms tested, Pseudomonas fluorescens significantly recorded maximum per cent mycelial growth reduction (41.90), sclerotial Number (70.65 per cent) and Sclerotial germination (58.67%) reduction over control. Among rhizosphere forty four Pseudomonas fluorescens and six isolates of Bacillus subtilis and Trichoderma sp 31 isolates tested for their antagonistic activity against Scerotium rolfsii by dual culture, one isolate namely T.viride (TVB 1) P. fluorescens (SBHRPF 2), B. subtilis (SBHRBS1) showed highest per cent mycelial growth inhibition allowed minimum of sclerotial production. Pot culture experiments The application of biocontrol agents triggered the activity of three defense related enzymes viz., peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were induced and the accumulation of phenol was also noticed in Tropical sugarbeet upon challenge inoculation soil application S.rolfsii the causal agent for root rot in tropical sugarbeet. The activities of defense enzymes reached a peak at four days after inoculation (DAI) with the pathogen. Native PAGE analysis revealed the expression of an additional isoforms of PO and PPO were observed in biocontrol agents treated soil application due to induced systemic resistance (ISR) induction.
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