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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2051-2066
DOI: https://doi.org/10.20546/ijcmas.2020.906.252


Induced Systemic Resistance by Rhizosphere and Phyllosphere Microorganism against Root Rot caused by Sclerotium rolfsii in Tropical Sugarbeet (TSB) Ecosystems
Paramasivan Mookkan* and N. Muthukrishnan
1(Plant Pathology), Dryland Agricultural Research Station, TNAU, Chettinad, TamilNadu, India
2(Agricultural Entomology), TamilNadu Agricultural University, Coimbatore, TamilNadu, India
*Corresponding author
Abstract:

Phylloplane and rhizosphere microorganism were isolated from tropical sugarbeet. Phyllolplane fungi viz., Cladosporum sp., Penicillium sp., Aspergillus niger, Aspergillus flavus, and Curvularia and Bacteria of B. subtilis and P. fluorescens were isolated seven phylloplane microorganisms tested, Pseudomonas fluorescens significantly recorded maximum per cent mycelial growth reduction (41.90), sclerotial Number (70.65 per cent) and Sclerotial germination (58.67%) reduction over control. Among rhizosphere forty four Pseudomonas fluorescens and six isolates of Bacillus subtilis and Trichoderma sp 31 isolates tested for their antagonistic activity against Scerotium rolfsii by dual culture, one isolate namely T.viride (TVB 1) P. fluorescens (SBHRPF 2), B. subtilis (SBHRBS1) showed highest per cent mycelial growth inhibition allowed minimum of sclerotial production. Pot culture experiments The application of biocontrol agents triggered the activity of three defense related enzymes viz., peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were induced and the accumulation of phenol was also noticed in Tropical sugarbeet upon challenge inoculation soil application S.rolfsii the causal agent for root rot in tropical sugarbeet. The activities of defense enzymes reached a peak at four days after inoculation (DAI) with the pathogen. Native PAGE analysis revealed the expression of an additional isoforms of PO and PPO were observed in biocontrol agents treated soil application due to induced systemic resistance (ISR) induction.


Keywords: Rhizosphere, Phylloplane, pseudomonas fluorescens and Induced systemic Resistance
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How to cite this article:

Paramasivan Mookkan and Muthukrishnan. N. 2020. Induced Systemic Resistance by Rhizosphere and Phyllosphere Microorganism against Root Rot caused by Sclerotium rolfsii in Tropical Sugarbeet (TSB) Ecosystems.Int.J.Curr.Microbiol.App.Sci. 9(6): 2051-2066. doi: https://doi.org/10.20546/ijcmas.2020.906.252