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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1923-1936
DOI: https://doi.org/10.20546/ijcmas.2020.906.238


Isolation and Identification of Periplasmic Proteins in Salmonella Typhimurium
Arijit Shome1*, Manoj Kumawat1,3, Pavan Kumar Pesingi2,4, Sanjeev Kumar Bhure1 and Manish Mahawar1
1Division of Biochemistry and
2Veterinary Public Health Indian Veterinary Research Institute, Izatnagar, UP 243 122 India
3ICMR- National Institute for Research in Environmental Health, Bhopal, MP 462030 India
4Faculty of Veterinary and Animal Sciences, BHU, Varanasi- India
*Corresponding author
Abstract:

Between the outer and inner membranes, Gram-negative bacteria contain a narrow space known as periplasm. Periplasm is very tiny spice; however, it is the home for many vital proteins required for various physiological functions as well as bacterial survival under stress conditions. In the current study, we have isolated and identified periplasmic proteins from enteric pathogen Salmonella Typhimurium. Based on the colonization pattern, first, we selected E-5591 as a virulent strain of Salmonella Typhimurium. Next, we isolated periplasmic proteins from E-5591 by modified osmotic shock method. The extraction was monitored by the estimation of malate dehydrogenase (MDH) activity. We have observed more than 90 % MDH activity (p< 0.001) in the cytoplasmic fraction. By LC-MS/MS, we have identified 117 periplasmic proteins and categorized them in 14 functional categories. Finally, we have discussed the role(s) of important proteins in bacterial physiology and virulence, with an emphasis on Salmonella Typhimurium.


Keywords: Salmonella Typhimurium, Periplasmic proteins
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How to cite this article:

Arijit Shome, Manoj Kumawat, Pavan Kumar Pesingi, Sanjeev Kumar Bhure and Manish Mahawar. 2020. Isolation and Identification of Periplasmic Proteins in Salmonella Typhimurium.Int.J.Curr.Microbiol.App.Sci. 9(6): 1923-1936. doi: https://doi.org/10.20546/ijcmas.2020.906.238