National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Between the outer and inner membranes, Gram-negative bacteria contain a narrow space known as periplasm. Periplasm is very tiny spice; however, it is the home for many vital proteins required for various physiological functions as well as bacterial survival under stress conditions. In the current study, we have isolated and identified periplasmic proteins from enteric pathogen Salmonella Typhimurium. Based on the colonization pattern, first, we selected E-5591 as a virulent strain of Salmonella Typhimurium. Next, we isolated periplasmic proteins from E-5591 by modified osmotic shock method. The extraction was monitored by the estimation of malate dehydrogenase (MDH) activity. We have observed more than 90 % MDH activity (p< 0.001) in the cytoplasmic fraction. By LC-MS/MS, we have identified 117 periplasmic proteins and categorized them in 14 functional categories. Finally, we have discussed the role(s) of important proteins in bacterial physiology and virulence, with an emphasis on Salmonella Typhimurium.
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