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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1824-1834
DOI: https://doi.org/10.20546/ijcmas.2020.906.227


Inbuilt Characteristics of Hydrolytic Enzymes Activities in Root Tissues of Chickpea (Cicer arietinum L) Cultivars of Gujarat against Fusarium Wilt (Fusarium oxysporium f.spp ciceri) Disease Infection
P. J. Rathod1* and D. N. Vakharia2
Department of Biochemistry, College of Agriculture, Junagadh Agricultural University, Junagadh-362001 (Gujarat) India
*Corresponding author
Abstract:

A field experiment was conducted In Sick plot of Pulse research station, JAU., Junagadh. To find out the genetic makeup of hydrolytic enzymes of six chickpea cultivars that was grown in normal (healthy soil) and sick plot (Diseased soil) in response to wilt disease. The pattern of theses enzymes showed The β -1,3-glucanase revealed that the significantly higher activity as the growth of plants and as the disease development, Plants grown in sick plot, the β -1, 3-glucanase activity in the different cultivars were varied between 89.02 to 368.42 µ mole glucose released.h-1.g-1.fr.wt. Susceptible cultivar JG-62 and GG-4 had significantly higher activity, than resistant and tolerant cultivars. Similarly In case of chitinase activity resulted significant differences in our experiments. The root tissues obtained from sick plot visualized higher chitinase activity as compared to the tissues received from normal plot. Among the cultivars, susceptible cultivars have similar trend as observed in β -1, 3- glucanase. Lower level of the enzyme activity in root tissues was observed in tolerant cultivars. Thus hypothesis on the basis of fungal invasion is also proved by hydrolytic enzymes and its can be classified as GG-1(tolerant), GG-2 (Tolerant), JG-62 (Highly susceptible), JCP-27, WR-315, (Highly Resistant) and GG-4 (Susceptible) to fusarium. It can be concluded that both β-1, 3-glucanase and chitinase activity having defensive role thus it can be used as biochemical marker for identification of fungal resistant cultivars.


Keywords: β -1,3- glucanase, Chitinase, Chickpea
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How to cite this article:

Rathod, P. J. and Vakharia, D. N. 2020. Inbuilt Characteristics of Hydrolytic Enzymes Activities in Root Tissues of Chickpea (Cicer arietinum L) Cultivars of Gujarat against Fusarium Wilt (Fusarium oxysporium f.spp ciceri) Disease Infection.Int.J.Curr.Microbiol.App.Sci. 9(6): 1824-1834. doi: https://doi.org/10.20546/ijcmas.2020.906.227