National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A field experiment was conducted In Sick plot of Pulse research station, JAU., Junagadh. To find out the genetic makeup of hydrolytic enzymes of six chickpea cultivars that was grown in normal (healthy soil) and sick plot (Diseased soil) in response to wilt disease. The pattern of theses enzymes showed The β -1,3-glucanase revealed that the significantly higher activity as the growth of plants and as the disease development, Plants grown in sick plot, the β -1, 3-glucanase activity in the different cultivars were varied between 89.02 to 368.42 µ mole glucose released.h-1.g-1.fr.wt. Susceptible cultivar JG-62 and GG-4 had significantly higher activity, than resistant and tolerant cultivars. Similarly In case of chitinase activity resulted significant differences in our experiments. The root tissues obtained from sick plot visualized higher chitinase activity as compared to the tissues received from normal plot. Among the cultivars, susceptible cultivars have similar trend as observed in β -1, 3- glucanase. Lower level of the enzyme activity in root tissues was observed in tolerant cultivars. Thus hypothesis on the basis of fungal invasion is also proved by hydrolytic enzymes and its can be classified as GG-1(tolerant), GG-2 (Tolerant), JG-62 (Highly susceptible), JCP-27, WR-315, (Highly Resistant) and GG-4 (Susceptible) to fusarium. It can be concluded that both β-1, 3-glucanase and chitinase activity having defensive role thus it can be used as biochemical marker for identification of fungal resistant cultivars.
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