International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:6, June, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1711-1713

Qualitative and Quantitative Biofilm Assay of Pseudomonas aeruginosa Isolates from Clinical Samples
Rekha Panwar1*, Virendra Gehlot2, Ramkumar1, Sunil Maherchandani1 and Basant1
1Department of Veterinary Microbiology and Biotechnology, University of Veterinary and Animal Sciences, Bikaner (Rajasthan), India
2Department of Dermatology and Venereology, Dr S N medical college, Jodhpur (Rajasthan), India
*Corresponding author

Pseudomonas aeruginosa is a Gram-negative bacterium found in multiple habitats, including soil, marine habitats and plants, can survive in harsh conditions such as high temperatures 42 °C and is capable of growing without oxygen. It is the fifth most commonly isolated organism and an opportunistic pathogen of animals. It is considered to be one of the major causes of nosocomial infections responsible for various clinical conditions such as pneumonia, urinary tract infections, and complications in clinical burns, wounds, and cystic fibrosis (CF). P. aeruginosa biofilm production is a well-known virulence factor and in the biofilm mode of growth it is involved in chronic as well as in acute infections. In present investigation 65 clinical sample of nosocomial infection taken from different animal species, namely dog, cattle camel, horse and buffalo. Total ten samples were found positive for Pseudomonas aeruginosa, which include four from Dog, one from Sheep, one from Goat, and four from Cattle whereas no samples were found positive for horse and buffalo. The aim of this study was to detect biofilm formation by study isolates of Pseudomonas aeruginosa qualitatively and quantitatively. Ten isolates were taken from diseased animal in veterinary clinics of RAJUVAS Bikaner. Qualitative biofilm formation assays (Pellicle formation assay and Congo red agar method) and quantitative assay by Microtitre plate assay used for biofilm production.

Keywords: Pseudomonas aeruginosa, Biofilm, Nosocomial infection

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Rekha Panwar, Virendra Gehlot, Ramkumar, Sunil Maherchandani and Basant. 2020. Qualitative and Quantitative Biofilm Assay of Pseudomonas aeruginosa Isolates from Clinical Samples.Int.J.Curr.Microbiol.App.Sci. 9(6): 1711-1713. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.