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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1563-1562
DOI: https://doi.org/10.20546/ijcmas.2020.906.193


Effect of Two Different Culture Media on Developmental Rate of Bovine Embryos invitro
S. R. Sanjeeva Kumar1, D. Reena2, S. Manokaran3* and S. Balasubramanian4
1Department of Animal Biotechnology, Madras Veterinary College, Chennai – 600 007, India
2Department of Clinics, Madras Veterinary College, Chennai – 600 007, India
3Kangayam Cattle Research Station,
Uppupallam, Baguthampalayam, Sathyamangalam – 638 451, India
4Tamil Nadu Veterinary and Animal Sciences University,
Madras Veterinary College campus, Chennai – 600 007, India
*Corresponding author
Abstract:

In the present study, effect of two different culture media on the developmental competence of bovine oocytes was studied. For the study a total of 1110 oocytes were collected using ovum pick-up (OPU) technique and the average oocyte yield was 8.34 ± 0.23 per OPU session per animal. The oocytes were graded as A, B, C and D grades based on the layers of compact cumulus cells surrounding the zona pellucida. Among this only A, B and C grade oocytes were used for in vitro maturation. The oocytes were matured in TCM 199 media supplemented with 10 per cent FBS, 1 mg/ml Folltropin (Bioniche, Canada), 0.02 IU/ml LH, 1 mg/ml estradiol with addition of 100 µM cysteamine, 10 ng/ml EGF, 100 ng/ml IGF, 10 µg/ml insulin, 5.5 µg/ml transferrin and 5 µg/ml selenium and the overall maturation rate was 91.30± 1.27 per cent.  The presumptive zygotes were cultured in two different culture media viz. i) two step sequential synthetic oviduct fluid (SOF) media and ii). single step potassium simplex optimised medium (KSOM). The mean (± SE) cleavage, 4 cell, 8 cell and morula development rate was 73.04 ± 1.50 and 79.01 ± 1.34 per cent, 54.88 ± 1.76 and  60.69 ± 1.89 per cent,  35.89 ± 1.57 and 44.67 ± 1.62 per cent and 19.18 ± 1.10 and 25.36 ± 1.37 per cent in SOF media and KSOM, respectively. Based on the cleavage and embryo development observed, it was concluded that KSOM could be better than SOF media for in vitro production of bovine embryos.


Keywords: Ovum pick-up, SOF media, KSOM, bovine embryo, in vitro culture
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How to cite this article:

Sanjeeva Kumar. G. S. R., D. Reena, S. Manokaran and Balasubramanian. S. 2020. Effect of Two Different Culture Media on Developmental Rate of Bovine Embryos invitro.Int.J.Curr.Microbiol.App.Sci. 9(6): 1563-1562. doi: https://doi.org/10.20546/ijcmas.2020.906.193