International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:6, June, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1515-1524

Assessment of Genetic Variability in Prolactin Gene and Its Impact on Milk Composition in Bos indicus Cattle
Vijay Kumar Agrawal1*, Gyanchand Gahlot1, Rajeev Joshi1, Govind Singh Dhakad1 and Sonal Thakur2
1Department of Animal Genetics and Breeding, Veterinary College, Bikaner (Raj.), India
2Department of Animal Husbandry, Govt. of Rajasthan, India
*Corresponding author

The candidate gene effect of prolactin gene (PRL) on milk traits suggests the important role of genetic variability in prolactin gene on milk composition parameters. Therefore the present study was conducted to explore the genetic variation in PRL gene through sequence based RFLP marker approach and to observe their association with milk composition in three Bos indicus cattle breeds (Rathi, Sahiwal and Kankrej). Genomic DNA was extracted from blood samples collected from 225 randomly selected milking cows maintained at Livestock Research Stations at Bikaner. A 156 bp fragment length of PRL gene was amplified through species specific primer. Milk samples from respective animals were analyzed on three different occasions for estimation of different constituents of milk. RFLP marker detected the presence of two genotypes; GG and AG in each breed. Sequence analysis identified an overall four nucleotide substitutions found to be responsible for genetic variation in PRL gene. Association analysis revealed the presence of significant association between genetic variability in prolactin gene and milk protein content with no impact on other milk constituents. The present study concluded that heterozygotes genotypes for PRL exon-3 region were favored in studied Bos indicus breeds to produce milk containing higher amount of protein.

Keywords: Prolactin, RFLP, Sequence polymorphism, Milk composition

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How to cite this article:

Vijay Kumar Agrawal, Gyanchand Gahlot1, Rajeev Joshi, Govind Singh Dhakad and Sonal Thakur. 2020. Assessment of Genetic Variability in Prolactin Gene and Its Impact on Milk Composition in Bos indicus Cattle.Int.J.Curr.Microbiol.App.Sci. 9(6): 1515-1524. doi:
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