National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The candidate gene effect of prolactin gene (PRL) on milk traits suggests the important role of genetic variability in prolactin gene on milk composition parameters. Therefore the present study was conducted to explore the genetic variation in PRL gene through sequence based RFLP marker approach and to observe their association with milk composition in three Bos indicus cattle breeds (Rathi, Sahiwal and Kankrej). Genomic DNA was extracted from blood samples collected from 225 randomly selected milking cows maintained at Livestock Research Stations at Bikaner. A 156 bp fragment length of PRL gene was amplified through species specific primer. Milk samples from respective animals were analyzed on three different occasions for estimation of different constituents of milk. RFLP marker detected the presence of two genotypes; GG and AG in each breed. Sequence analysis identified an overall four nucleotide substitutions found to be responsible for genetic variation in PRL gene. Association analysis revealed the presence of significant association between genetic variability in prolactin gene and milk protein content with no impact on other milk constituents. The present study concluded that heterozygotes genotypes for PRL exon-3 region were favored in studied Bos indicus breeds to produce milk containing higher amount of protein.
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