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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1380-1387
DOI: https://doi.org/10.20546/ijcmas.2020.906.170


Assessment of Invitro Differentiation Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells to Skeletal Myocytes
R. S. Sandhu1*, N. S. Jadon1, P. Pandey2, D. Bodh1, P. Joshi1, M. Kandpal1, R. Saini 1 and A. Kumar1
1Deptartmen of Veterinary Surgery & Radiology,
2Department of MBGE, CBSH, G.B.P.U.A. and T., Pantnagar (U.S.Nagar), Uttarakhand (Pin: 263145), India
*Corresponding author
Abstract:

Mesenchymal stem cells are multipotent/pluripotent stromal cells that can differentiate into a diversity of cell categories depending upon the micro-environment to that they are exposed. The multipotent differentiation potential of MSCs is generally towards a constrained sum of cell types familiar to the tissue of origin and maintains the capacity for self-renewal. To assess the differentiation potential of MSCs to skeletal myocytes a male mongrel dog of 3 years was used to carry out the research. The marrow derived mesenchymal stem cells were harvested with mononuclear cells and cultivated in monolayer culture in T-25 tissue culture flasks till they attained of 80-90 % confluency. At 4th passage the cells were driven towards skeletal myogenesis by addition of skeletal myocytes differentiation medium containing 5-azacytidine, foetal horse serum, basic fibroblast growth factor, dexamethasone and hydrocortisone. The cells which differentiated showed positive results of RT-PCR for the early myogenic differentiation factors Myf5 and Myogenin and Hoechst 33342 staining. The Hoechst 33342 stainin, RT-PCR analysis and scanning electron microscopy leads to the positive result for differentiated skeletal myocytes in vitro. The differentiated skeletal myocytes can be used in tissue engineering and veterinary therapeutics for reparing the damaged skeletal myocytes.


Keywords: MSCs, Skeletal myocytes, Myf5, Myogenin, Hoechst 33342.
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How to cite this article:

Sandhu. R. S., N. S. Jadon, P. Pandey, D. Bodh, P. Joshi, M. Kandpal, R. Saini and Kumar. A. 2020. Assessment of Invitro Differentiation Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells to Skeletal Myocytes.Int.J.Curr.Microbiol.App.Sci. 9(6): 1380-1387. doi: https://doi.org/10.20546/ijcmas.2020.906.170