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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1372-1379
DOI: https://doi.org/10.20546/ijcmas.2020.906.169


Molecular Screening for MYMV Resistance in Interspecific Hybrids of Vigna raidiata with Wild Vigna sp. using Tobacco N Gene Primers
P. Sivakumar1*, M. Pandiyan2 and A. Manickam3
1Department of Crop Improvement, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Eachangkottai, Thanjavur-614 902, India
2Department of Crop Improvement, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Vazhavachanur, Thiruvannamalai - 606 753, India
3Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore-641 003, India
*Corresponding author
Abstract:

Interspecific hybridization was made between mungbean yellow mosaic virus (MYMV) susceptible greengram variety CO 5 with MYMV resistant wild Vigna sp. Crossed seeds were raised in field condition for testing the MYMV incidence. F1 showed dominant resistance reaction against MYMV disease incidence. The field growing F2 populations of C0 5 x wild Vigna sp, cross produced a wide range of segregant viz., susceptible, parental and intermediate type. F 2progenies were subjected to PCR amplification using tobacco N gene primers designed based on the tobacco N-gene and Arabidopsis RPS gene sequences. The PCR reaction was carried out and expected product was amplified at annealing temperature at 40°C. In parental polymorphisms, a detectable single band of 500bp being present in MYMV resistant Wild Vigna species, which was absent in MYMV susceptible CO 5 greengram variety. All the F2 resistant individuals showed 500bp DNA band with use of the same tobacco N gene primers.


Keywords: Mungbean, MYMV resistance, Tobacco N gene primers, Inter specific hybrids
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How to cite this article:

Sivakumar. P. M., Pandiyan and Manickam. A. 2020. Molecular Screening for MYMV Resistance in Interspecific Hybrids of Vigna raidiata with Wild Vigna sp. using Tobacco N Gene Primers.Int.J.Curr.Microbiol.App.Sci. 9(6): 1372-1379. doi: https://doi.org/10.20546/ijcmas.2020.906.169