National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Interspecific hybridization was made between mungbean yellow mosaic virus (MYMV) susceptible greengram variety CO 5 with MYMV resistant wild Vigna sp. Crossed seeds were raised in field condition for testing the MYMV incidence. F1 showed dominant resistance reaction against MYMV disease incidence. The field growing F2 populations of C0 5 x wild Vigna sp, cross produced a wide range of segregant viz., susceptible, parental and intermediate type. F 2progenies were subjected to PCR amplification using tobacco N gene primers designed based on the tobacco N-gene and Arabidopsis RPS gene sequences. The PCR reaction was carried out and expected product was amplified at annealing temperature at 40°C. In parental polymorphisms, a detectable single band of 500bp being present in MYMV resistant Wild Vigna species, which was absent in MYMV susceptible CO 5 greengram variety. All the F2 resistant individuals showed 500bp DNA band with use of the same tobacco N gene primers.
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