National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacterial leaf spot caused by Xanthomonas cucurbitae is one of the important diseases of bottle gourd and pumpkin and is gaining momentum in the sub-tropical zone of Himachal Pradesh. Studies were conducted under artificial inoculation conditions to standardize the inoculum concentration and method of inoculation for the development of disease. The bacterial concentration was standardized with the help of a growth curve of both bottle gourd and pumpkin isolates of X cucurbitae with time and a sigmoid curve was obtained in both the isolates in which the bacterial concentration reached its maximum (107 cfu/ml) after 96 days of inoculation and then declined. Studies on effect of different inoculum densities of causal bacterium on disease development revealed that an inoculum concentration of 108 cfu/ml of both the isolates resulted in minimum incubation period (2 and 4 days) and maximum mean disease severity (38.89 and 42.00%) in both bottle gourd and pumpkin plants, respectively. Among various methods of inoculation with both isolates, syringe method of inoculation resulted in minimum incubation period (2.33 and 3.33 days) and maximum disease severity (46.33 and 37.97 %) in both bottle gourd and pumpkin, respectively followed by pin prick method of inoculation.
Citations |
 |
 |
 |
 |
