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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1358-1371
DOI: https://doi.org/10.20546/ijcmas.2020.906.168


Effect of Inoculum Density and Methods of Inoculation on the Development of Bacterial Leaf Spot of Bottle Gourd and Pumpkin caused by Xanthomonas cucurbitae
Sujata Kumari1, Kumud Jarial2* and R. S. Jarial3
Department of Plant Pathology, College of Horticulture and Forestry, Neri, Hamirpur (HP) -177001, India
*Corresponding author
Abstract:

Bacterial leaf spot caused by Xanthomonas cucurbitae is one of the important diseases of bottle gourd and pumpkin and is gaining momentum in the sub-tropical zone of Himachal Pradesh. Studies were conducted under artificial inoculation conditions to standardize the inoculum concentration and method of inoculation for the development of disease. The bacterial concentration was standardized with the help of a growth curve of both bottle gourd and pumpkin isolates of X cucurbitae with time and a sigmoid curve was obtained in both the isolates in which the bacterial concentration reached its maximum (107 cfu/ml) after 96 days of inoculation and then declined. Studies on effect of different inoculum densities of causal bacterium on disease development revealed that an inoculum concentration of 108 cfu/ml of both the isolates resulted in minimum incubation period (2 and 4 days) and maximum mean disease severity (38.89 and 42.00%) in both bottle gourd and pumpkin plants, respectively. Among various methods of inoculation with both isolates, syringe method of inoculation resulted in minimum incubation period (2.33 and 3.33 days) and maximum disease severity (46.33 and 37.97 %) in both bottle gourd and pumpkin, respectively followed by pin prick method of inoculation.


Keywords: Bacterial leaf spot, Xanthomonas cucurbitae, inoculum density, bottle gourd and pumpkin
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How to cite this article:

Sujata Kumari, Kumud Jarial and Jarial. R. S. 2020. Effect of Inoculum Density and Methods of Inoculation on the Development of Bacterial Leaf Spot of Bottle Gourd and Pumpkin caused by Xanthomonas cucurbitae.Int.J.Curr.Microbiol.App.Sci. 9(6): 1358-1371. doi: https://doi.org/10.20546/ijcmas.2020.906.168