International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:6, June, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1346-1357

Detection of Bio-film Production among the Most Frequent Bacterial Isolates in Cases of Chronic Suppurative Otitis Media: A Cross-sectional Study
Priya Bhat1, Sameer Peer2* and Raksha Yoganand1
1Department of Microbiology, Employees State Insurance Corporation Medical College and Hospital, Rajajinagar, Bengaluru - 560010, Karnataka, India
2Department of Neuroimaging and interventional Radiology, National Institute of Mental Health and Neurosciences, Bengaluru-560029, Karnataka, India
*Corresponding author

To detect biofilm production among the most common micro-organisms isolated from CSOM cases. Five hundred consecutive patients with a diagnosis of CSOM were enrolled in the study. Organisms were isolated using standard culture methods and biochemical reactions. Antibiotic sensitivity pattern was determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Biofilm production was detected using Tube method and microtitre plate method. The most common organism isolated was Pseudomonas aeruginosa in 220 cases(44%) followed by Staphylococcus aureus in 110 cases (22%). Out of 186 isolates tested for biofilm production, 149 isolates (80%) showed biofilm production. Out of the moderately positive and strongly positive biofilm producers, 90% isolates were from unsafe CSOM. High proportion of isolates from CSOM cases shows biofilm production. Biofilm production may account for antibiotic resistance, recurrence of CSOM and failure of surgical procedures in CSOM cases.

Keywords: Biofilm, Antibiotic, Chronic Suppurative Otitis Media, Tympanoplasty

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How to cite this article:

Priya Bhat, Sameer Peer and Raksha Yoganand. 2020. Detection of Bio-film Production among the Most Frequent Bacterial Isolates in Cases of Chronic Suppurative Otitis Media: A Cross-sectional Study.Int.J.Curr.Microbiol.App.Sci. 9(6): 1346-1357. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.