National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To detect biofilm production among the most common micro-organisms isolated from CSOM cases. Five hundred consecutive patients with a diagnosis of CSOM were enrolled in the study. Organisms were isolated using standard culture methods and biochemical reactions. Antibiotic sensitivity pattern was determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Biofilm production was detected using Tube method and microtitre plate method. The most common organism isolated was Pseudomonas aeruginosa in 220 cases(44%) followed by Staphylococcus aureus in 110 cases (22%). Out of 186 isolates tested for biofilm production, 149 isolates (80%) showed biofilm production. Out of the moderately positive and strongly positive biofilm producers, 90% isolates were from unsafe CSOM. High proportion of isolates from CSOM cases shows biofilm production. Biofilm production may account for antibiotic resistance, recurrence of CSOM and failure of surgical procedures in CSOM cases.
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