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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1271-1279
DOI: https://doi.org/10.20546/ijcmas.2020.906.157


Expression of Heat Shock Protein 70-1 in Peripheral Blood Mononuclear Cells of Jersey-Sahiwal Cross Breds during Thermal Stress
V. Manasa*, T. Venkata Sai Kumar and T. Prasada Rao
Department of Veterinary Biochemistry, SVVU College of Veterinary Science, Proddatur, AP-516360, India
*Corresponding author
Abstract:

Heat Shock Protein 70 (HSP70) is one of the important molecular chaperon that can protect cells from thermal damage by involving in the maintenance of the intra cellular homeostasis primarily by controlling the process of protein folding. The indigenous breeds of cattle have been found to be more resistant to heat stress in comparison to the exotic breeds by way of expressing HSP70 as an adaptive response. Therefore, the present study was done to study the expression of HSP 70-1 in peripheral blood mononuclear cells (PBMCs) of Jersey- Sahiwal cross breds during thermal stress. Blood samples collected from the animals were used for the separation of PBMCs by using Histopaque 1077 and RBC lysis buffer. Total RNA was isolated from the separated PBMCs using Trizol reagent. The isolated total RNA was then reverse transcribed to cDNA that was used for amplification of HSP70-1 gene using specific pair of primers. The presence of 1926 base pair band in denaturing agarose indicated the amplification of HSP70-1 gene. To conclude, our results demonstrate that HSP70-1 gene is expressed in PBMCs of Jersey- Sahiwal cross breds and further characterization of this gene may help in elucidating its role in attributing thermo tolerance to the cross bred cattle.


Keywords: HSP70-1 gene, Peripheral Blood Mononuclear Cells, Jersey- Sahiwal Cross Breds, Thermal Stress,
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How to cite this article:

Manasa. V., T. Venkata Sai Kumar and Prasada Rao. T. 2020. Expression of Heat Shock Protein 70-1 in Peripheral Blood Mononuclear Cells of Jersey- Sahiwal Cross Breds during Thermal Stress.Int.J.Curr.Microbiol.App.Sci. 9(6): 1271-1279. doi: https://doi.org/10.20546/ijcmas.2020.906.157