National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Heat Shock Protein 70 (HSP70) is one of the important molecular chaperon that can protect cells from thermal damage by involving in the maintenance of the intra cellular homeostasis primarily by controlling the process of protein folding. The indigenous breeds of cattle have been found to be more resistant to heat stress in comparison to the exotic breeds by way of expressing HSP70 as an adaptive response. Therefore, the present study was done to study the expression of HSP 70-1 in peripheral blood mononuclear cells (PBMCs) of Jersey- Sahiwal cross breds during thermal stress. Blood samples collected from the animals were used for the separation of PBMCs by using Histopaque 1077 and RBC lysis buffer. Total RNA was isolated from the separated PBMCs using Trizol reagent. The isolated total RNA was then reverse transcribed to cDNA that was used for amplification of HSP70-1 gene using specific pair of primers. The presence of 1926 base pair band in denaturing agarose indicated the amplification of HSP70-1 gene. To conclude, our results demonstrate that HSP70-1 gene is expressed in PBMCs of Jersey- Sahiwal cross breds and further characterization of this gene may help in elucidating its role in attributing thermo tolerance to the cross bred cattle.
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