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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 1197-1203
DOI: https://doi.org/10.20546/ijcmas.2020.906.149


Identification of Species of Meat Origin using ATPase Gene Variability by the Polymerase Chain Reaction
P. P. Bhavsar1*, M. N. Brahmbhatt2, J. B. Nayak2, B. C. Parmar2, J. H. Chaudhary2, H. K. Gida2 and D. M. Paghdar2
1Department of Livestock Products Technology,
2Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
*Corresponding author
Abstract:

Food authenticity is currently an issue of major concern for food authorities, since incorrect labelling of animal foods may have remarkable negative consequences. To circumvent this problem, DNA based method had been utilised. The present study was carried out for the detection of meat species by using variability in ATPase Subunit 6 and 8 genes by multiplex PCR. Meat samples from cattle, buffalo, sheep, goat, chicken and dog were utilized for molecular analysis. KAPA express extract kit was used to extract DNA from meat samples. Sequences among mitochondrial ATPase Subunit 6 and 8 genes were targeted for species-specific amplifications. The specificity of the primers was checked by using the Basic Local Alignment Search Tool (BLAST) software. The designed primers yielded specific amplification of 65, 107, 144, 186, 200 and 232 bp for Goat, Sheep, Buffalo, Dog, Poultry and Cattle respectively. Further to detect the sensitivity of the assay different level of meat mixture of 16, 8, 4, 2, 1 and 0.1% was formulated by adding pork. The assay successfully identified the presence of meat at the level of 1%. In the current assay, cooked and putrefied meat samples also showed successful amplification of the DNA, so this assay is useful in the detection of meat adulteration.


Keywords: Animal foods, DNA, meat Species, ATPase
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How to cite this article:

Bhavsar. P. P., M. N. Brahmbhatt, J. B. Nayak, B. C. Parmar, J. H. Chaudhary, H. K. Gida and Paghdar. D. M. 2020. Identification of Species of Meat Origin using ATPase Gene Variability by the Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 9(6): 1197-1203. doi: https://doi.org/10.20546/ijcmas.2020.906.149