National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The samples collected for comparison of the three techniques, viz., cytobrush (CB), uterine lavage (LVL) and endometrial biopsy (EB) from 10 mares with endometritis were evaluated by cytological, bacteriological and histological methods. A total of 6 (60 %) mares had positive histopathology for endometritis. The results of cytological examinations were compared for the presence of infiltration by PMNs of the luminal epithelium and stratum compactum as histological examination of the endometrium is generally used as the reference standard for diagnosis of endometritis. Positive growth from EB was obtained in 7 (70 %) of all mares, from CB in 6 (60 %) and from LVL in 8 (80 %) mares. No statistical differences in the number of positive cultures were obtained between different techniques. In 3 of 10 (30 %) cytological smears obtained from EB and CB and in 4 of 10 (40 %) cytological smears obtained from LVL, the percentage of PMNs was ≥ 1. This difference was not significant. In EB, CB and LVL, all the cytologically positive mares also had positive cultures. Whereas, 30 % positive cultures from CB and 40 % positive cultures from EB and LVL had negative cytology. The most common isolated bacteria were β-hemolytic Streptococci and E. coli either alone (30 %) or in combination in all three techniques. There were no differences in the number of positive cultures between CB, EB and LVL or in positive cytology findings between the three methods. Isolation of β-hemolytic Streptococci from positive cytology was indicative of their pathogenicity. The findings however warrant further study on larger sample size on mares with clinical and subclinical endometritis.
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