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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 555-562
DOI: https://doi.org/10.20546/ijcmas.2020.906.072


Association of Cytology, Microbiology and Histopathology of Cytobrush, Uterine Lavage and Endometrial Biopsy Samples from Mares with Endometritis
Agnus Mathew1*, D. M. Patel2, K. K. Hadiya1, B. B. Bhanderi3 and D. J. Ghodasara4
1Department of Veterinary Gynaecology & Obstetrics,
2Dept. of Veterinary Clinical Complex,
 3Department of Veterinary Microbiology,
4Department of Veterinary Pathology, College of Veterinary Science and AH, Anand Agril University, Anand-388 001, India
*Corresponding author
Abstract:

The samples collected for comparison of the three techniques, viz., cytobrush (CB), uterine lavage (LVL) and endometrial biopsy (EB) from 10 mares with endometritis were evaluated by cytological, bacteriological and histological methods. A total of 6 (60 %) mares had positive histopathology for endometritis. The results of cytological examinations were compared for the presence of infiltration by PMNs of the luminal epithelium and stratum compactum as histological examination of the endometrium is generally used as the reference standard for diagnosis of endometritis. Positive growth from EB was obtained in 7 (70 %) of all mares, from CB in 6 (60 %) and from LVL in 8 (80 %) mares. No statistical differences in the number of positive cultures were obtained between different techniques. In 3 of 10 (30 %) cytological smears obtained from EB and CB and in 4 of 10 (40 %) cytological smears obtained from LVL, the percentage of PMNs was ≥ 1. This difference was not significant. In EB, CB and LVL, all the cytologically positive mares also had positive cultures. Whereas, 30 % positive cultures from CB and 40 % positive cultures from EB and LVL had negative cytology. The most common isolated bacteria were β-hemolytic Streptococci and E. coli either alone (30 %) or in combination in all three techniques. There were no differences in the number of positive cultures between CB, EB and LVL or in positive cytology findings between the three methods. Isolation of β-hemolytic Streptococci from positive cytology was indicative of their pathogenicity. The findings however warrant further study on larger sample size on mares with clinical and subclinical endometritis.


Keywords: Endometritis, Mare, Cytobrush, Endometrial biopsy, Low volume uterine lavage, Bacteriology, Cytology, Histopathology.
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How to cite this article:

Agnus Mathew, D. M. Patel, K. K. Hadiya, B. B. Bhanderi and Ghodasara. D. J. 2020. Association of Cytology, Microbiology and Histopathology of Cytobrush, Uterine Lavage and Endometrial Biopsy Samples from Mares with Endometritis.Int.J.Curr.Microbiol.App.Sci. 9(6): 555-562. doi: https://doi.org/10.20546/ijcmas.2020.906.072