National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pigeon pea is an important pulse crop of the world which is an important source of protein and other nutrients. Phytophthora leaf blight is a major disease of pigeon pea which is caused by Phytophthora drechsleri f.sp. cajani. In vitro evaluation of different isolates of Trichoderma and a strain of Pseudomonas fluorescens against Phytophthora drechsleri f.sp. cajani was carried out to test the efficiency of these antagonists as biocontrol agents against the test pathogen. Ten isolates of Trichoderma spp. and Pseudomonas fluorescens were tested against the blight pathogen under dual culture experiments. Volatile and non-volatile metabolites released from the antagonists were also tested against the pathogen. Percent inhibition in the mycelia growth of the pathogen was determined. All Trichoderma isolates showed significant inhibitory effect on the pathogen in all the experiments. Under dual culture method maximum and minimum percentage of inhibition was showed by AN-33 (81.7%) and AN-37 (63.4%) respectively. Under volatile experiments AN-48 (62.6%) and AN-6 (46.9%) showed maximum and minimum percent inhibition respectively. Under non-volatile experiments, the highest inhibition of pathogen was observed at 15% concentration of the culture filtrates of the bio agents. At this concentration AN-33 exhibited the maximum (72%) and AN-6 showed the minimum (43.5%) inhibition to the pathogen growth. Pseudomonas fluorescens showed significant inhibition in the mycelial growth of the pathogen under dual culture test with percentage inhibition of 43.02%. The study suggests that the Trichoderma spp. has a potential to be used as biocontrol agents against Phytophthora blight of pigeon pea. Trichoderma isolate AN-33 was identified as the most effective isolate out of the ten isolates studied.
Citations |
 |
 |
 |
 |
