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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 3536-3543
DOI: https://doi.org/10.20546/ijcmas.2020.905.421


Evaluation of Biocontrol Agents against Phytophthora drechsleri F.sp. cajani (Leaf Blight) in Pigeonpea
Sujata Singh Yadav*, Someshwar Bhagat, Vishal Singh, Shahnashi Hashmi and Meghraj Bhagel
National Research Centre for Integrated Pest Management,  Indian Council of Agricultural Research, India Institute of Agriculture Sciences, Bundelkhand University Campus, Jhansi, India
*Corresponding author
Abstract:

Pigeon pea is an important pulse crop of the world which is an important source of protein and other nutrients. Phytophthora leaf blight is a major disease of pigeon pea which is caused by Phytophthora drechsleri f.sp. cajani. In vitro evaluation of different isolates of Trichoderma and a strain of Pseudomonas fluorescens against Phytophthora drechsleri f.sp. cajani was carried out to test the efficiency of these antagonists as biocontrol agents against the test pathogen. Ten isolates of Trichoderma spp. and Pseudomonas fluorescens were tested against the blight pathogen under dual culture experiments. Volatile and non-volatile metabolites released from the antagonists were also tested against the pathogen. Percent inhibition in the mycelia growth of the pathogen was determined. All Trichoderma isolates showed significant inhibitory effect on the pathogen in all the experiments. Under dual culture method maximum and minimum percentage of inhibition was showed by AN-33 (81.7%) and AN-37 (63.4%) respectively. Under volatile experiments AN-48 (62.6%) and AN-6 (46.9%) showed maximum and minimum percent inhibition respectively. Under non-volatile experiments, the highest inhibition of pathogen was observed at 15% concentration of the culture filtrates of the bio agents. At this concentration AN-33 exhibited the maximum (72%) and AN-6 showed the minimum (43.5%) inhibition to the pathogen growth. Pseudomonas fluorescens showed significant inhibition in the mycelial growth of the pathogen under dual culture test with percentage inhibition of 43.02%. The study suggests that the Trichoderma spp. has a potential to be used as biocontrol agents against Phytophthora blight of pigeon pea. Trichoderma isolate AN-33 was identified as the most effective isolate out of the ten isolates studied.


Keywords: Phytophthora, Trichoderama, Pseudomonas, antagonist, volatile, non-volatile compounds
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How to cite this article:

Sujata Singh Yadav, Someshwar Bhagat, Vishal Singh, Shahnashi Hashmi and Meghraj Bhagel. 2020. Evaluation of Biocontrol Agents against Phytophthora drechsleri F.sp. cajani (Leaf Blight) in Pigeonpea.Int.J.Curr.Microbiol.App.Sci. 9(5): 3536-3543. doi: https://doi.org/10.20546/ijcmas.2020.905.421