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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 3420-3427
DOI: https://doi.org/10.20546/ijcmas.2020.905.406


Evaluation of Sperm Motility with Glutathione and Honey in Skim Milk based Extenders by CASA in Boer Buck
Suresh Arakeri1*, M. K. Tandle2, M. D. Suranagi2, Vinay Tikare2, R. G. Bijurkar2, Jagannath Rao2 and Shrikant Kulkarni2
1Veterinary Officer, Dharwad, Karnataka, India
2KVAFSU, Bidar, Karnataka, INDIA
*Corresponding author
Abstract:

The aim of the study was to evaluate and compare Boer buck sperm motility with 5 mM Glutathione (G) and or 1% or 2% Honey (H) in Skim milk (SM) based extenders preserved at refrigeration temperature at 0, 24, 48 and 72 hrs. A total of 72 ejaculates were collected equally from 6 mature bucks at weekly interval by using Artificial Vagina (AV) as per standard procedure. All the ejaculates were diluted using six Skim milk based extenders viz. SME, SMGE, SMGH(1%)E, SMGH(2%)E, SMH(1%)E and SMH(2%)E. The sperm motility was evaluated by CASA (Computer Assisted Semen Analyzer). The data obtained was statistically analyzed by General Linear Model (GLM) and procedure. The results showed that sperm motility was differed significantly (P<0.05) from extender to extender at 24 h, 48 h and 72 h of refrigeration. Based on the results concluded that supplementation of optimum concentration of glutathione (5 mM) and honey (1%) maintained better sperm motility upto 72 hours of storage and successful preservation of buck spermatozoa at refrigeration temperature. Hence study revealed that Boer buck semen can be preserved effectively with SMGH(1%)E, at refrigeration temperature upto 72 hours of storage.


Keywords: Glutathione, Honey, Skim milk, Buck, Sperm, CASA
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How to cite this article:

Suresh Arakeri, M. K. Tandle, M. D. Suranagi, Vinay Tikare, R. G. Bijurkar, Jagannath Rao and Shrikant Kulkarni. 2020. Evaluation of Sperm Motility with Glutathione and Honey in Skim Milk based Extenders by CASA in Boer Buck.Int.J.Curr.Microbiol.App.Sci. 9(5): 3420-3427. doi: https://doi.org/10.20546/ijcmas.2020.905.406