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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 1721-1730
DOI: https://doi.org/10.20546/ijcmas.2020.905.193


Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Genetic Diversity Analysis in Different Varieties of Roses (Rosa spp.)
Tanay Joshi*, Santosh Kumar, Mohan Bhimte and Lakhapati Singh
Department of International Cooperation, Research Institute of Organic Agriculture FiBL, Switzerland
*Corresponding author
Abstract:

Characterization and quantification of diversity in plant genetic resources were important to identify cultivars and clones, decipher relationships including parentages, and to efficiently manage germplasm collections. The present studies was undertaken to find out genetic diversity among twenty-one genotypes of rose (Rosa species) using EST-SSR markers. Out of the total 35 EST-SSR primers screened, a set of 12 primers showed polymorphism. PCR amplification yielded a total of 31 amplified products with a range 2 and 4 bands per primer (amplified size 90bp -550bp). The polymorphic information content (PIC)value of the primers ranged from (0.22- 0.96) with Highest PIC and Marker Index (MI) was recorded by primers RS-18 followed by Primer RS-30 and RS-10. Hence, primer RS-18 and Primer RS-30 were found to be highly informative. Jaccard’s similarity coefficient ranged from 0.143 to 0.923. Minimum similarity was observed between the genotype (Lady X) and (Happiness) (0.143). The similarity coefficient used to construct a dendrogram using UPGMA cluster analysis. The dendrogram grouped the twenty-one rose genotypes into three distinct clusters. Therefore, from this study the genotypes (Lady X) and (Happiness) (0.143) possesses diverse genetic background which can also be used as parent for further hybridization programme.


Keywords: EST-SSR, Rosa spp, Dendrogram, Polymorphic information content
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How to cite this article:

Tanay Joshi, Santosh Kumar, Mohan Bhimte and Lakhapati Singh. 2020. Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Genetic Diversity Analysis in Different Varieties of Roses (Rosa spp.).Int.J.Curr.Microbiol.App.Sci. 9(5): 1721-1730. doi: https://doi.org/10.20546/ijcmas.2020.905.193