National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
DEHP (di-ethyl hexyl phthalate) is widely used as a plasticizer and it adversely affects humans. In this study, one of the promising DEHP-degrading strain T28 had been selected among isolated pure bacterial strain from rubbish landfill soil near the hospital area, Patna. The bacterial strain T28 was capable of consuming DEHP as a sole source of carbon and energy. By analysing morphological, biophysical, biochemical characteristics, gram staining technique followed by the analysis of 16s rRNA phylogenetic studies, the strain was identified as Bacillus subtilis (Genbank Accession no. CPO230367). The mechanism of biodegradation and its characterization at different environmental parameters influencing the degradation process in contaminated soil have also been examined. The results of this study showed the optimal pH value ranges (7.0-8.0) optimal temperature ranges (37-50 ÌŠ C) while salinity could tolerate up to 5% which influenced the degradation rate in soil. We also investigated the effect of different carbon sources, nitrogen sources and different DEHP concentrations on its degradation. The isolated strain Bacillus subtilis degraded more than 99% of 250mg/l of DEHP within 24-48 hrs. The metabolites or end products were detected by liquid chromatography and mass spectrometry (LCMS) analysis. We hope that these findings can provide some information in the bioremediation of DEHP from contaminated soil.
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