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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 1499-1507
DOI: https://doi.org/10.20546/ijcmas.2020.905.170


Screening of Di-2-Ethyl Hexyl Phthalate (DEHP) Degrading Bacteria and its Characterization by Liquid Chromatography Mass Spectrometry Analysis
Madhavi Rashmi1*, Sonal Suman1 and Tanuja2
1Department of Botany & Biotechnology, T.P.S College,
Patliputra University, Patna- 800020, Bihar, India
2Department of Botany, Patliputra University, Patna-800020, Bihar, India
*Corresponding author
Abstract:

DEHP (di-ethyl hexyl phthalate) is widely used as a plasticizer and it adversely affects humans. In this study, one of the promising DEHP-degrading strain T28 had been selected among isolated pure bacterial strain from rubbish landfill soil near the hospital area, Patna. The bacterial strain T28 was capable of consuming DEHP as a sole source of carbon and energy. By analysing morphological, biophysical, biochemical characteristics, gram staining technique followed by the analysis of 16s rRNA phylogenetic studies, the strain was identified as Bacillus subtilis (Genbank Accession no. CPO230367). The mechanism of biodegradation and its characterization at different environmental parameters influencing the degradation process in contaminated soil have also been examined. The results of this study showed the optimal pH value ranges (7.0-8.0) optimal temperature ranges (37-50 ̊ C) while salinity could tolerate up to 5% which influenced the degradation rate in soil. We also investigated the effect of different carbon sources, nitrogen sources and different DEHP concentrations on its degradation. The isolated strain Bacillus subtilis degraded more than 99% of 250mg/l of DEHP within 24-48 hrs. The metabolites or end products were detected by liquid chromatography and mass spectrometry (LCMS) analysis. We hope that these findings can provide some information in the bioremediation of DEHP from contaminated soil.


Keywords: Plasticizer, DEHP; Biodegradation; phylogenetic studies, environmental parameters, LCMS analysis
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How to cite this article:

Madhavi Rashmi, Sonal Suman and Tanuja. 2020. Screening of Di-2-Ethyl Hexyl Phthalate (DEHP) Degrading Bacteria and its Characterization by Liquid Chromatography Mass Spectrometry Analysis.Int.J.Curr.Microbiol.App.Sci. 9(5): 1499-1507. doi: https://doi.org/10.20546/ijcmas.2020.905.170