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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 1800-1805
DOI: https://doi.org/10.20546/ijcmas.2020.905.204


Characterization of Leaf Curl Disease of Tomato Grown in Karnataka
Priyanka1*, M. S. Patil1, G. Uday2, Narayan S. Moger3 and C. Channakeshava4
1Department of Plant Pathology, 2Department of Genetics and Plant Breeding,
3Institute of Agricultural Biotechnology, University of Agricultural Sciences, Dharwad 580005, (Karnataka), India
4Division of Plant Pathology, ICAR-Indian Institute of Horticultural Research,
Hesaraghatta Lake Post, Bengaluru 560089, (Karnataka), India
*Corresponding author
Abstract:

During survey, tomato leaf samples showing leaf curl symptoms were collected from different tomato fields of Dharwad, Haveri and Kolar districts. Total nucleic acid extraction was carried out by cetyl trimethyl ammonium bromide (CTAB) method and quantified to perform polymerase chain reaction using Begomovirus group specific coat protein primers targeting the coat protein region and were sequenced. The nucleotide sequences were analyzed using the BLAST tool for which results indicated the presence of Tomato leaf curl virus (ToLCV), Croton yellow vein mosaic virus (CYVMV) and Chilli leaf curl virus (ChiLCV) that shows the association of these viruses in leaf curl symptoms in tomato obtained from the fields. The cluster analysis for all the ten sequences showed the grouping of the Chilli leaf curl virus and Tomato leaf curl virus into separate groups. Besides, the CYVMV was sub-grouped in ToLCV group. Thus this study shows the occurrence of different Begomoirus in leaf curl symptoms associated tomato growing in different regions of Karnataka.


Keywords: Tomato, Leaf curl, Begomovirus, ToLCV, ChiLCV, CYVMV
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How to cite this article:

Priyanka, K., M. S. Patil, G. Uday, Narayan S. Moger and Channakeshava, C. 2020. Characterization of Leaf Curl Disease of Tomato Grown in Karnataka.Int.J.Curr.Microbiol.App.Sci. 9(5): 1800-1805. doi: https://doi.org/10.20546/ijcmas.2020.905.204