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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 944-951
DOI: https://doi.org/10.20546/ijcmas.2020.905.104


Detection and Molecular Characterization of Food Borne Pathogens from Ready to Eat Meat and Milk Products
Rathnapraba1*, B. J. Shathish Sharma2, P. Kanagaraju3, Meignanalakshmiand S. Manoharan5
1Vaccine Research Centre, Viral Vaccines, Centre for Animal Health Studies,
TANUVAS, Madhavaram Milk Colony, Chennai-51, India
2Globion India Private Ltd., Hyderabad, India
3Livestock Farm Complex, VC&RI, Tirunelveli 627 358, India
4Department of Animal Biotechnology, MVC, Chennai, India
5Vaccine Research Centre, Bacterial Vaccines, Centre for Animal Health Studies,
TANUVAS, Madhavaram Milk Colony, Chennai-51, India
*Corresponding author
Abstract:

Foodborne illness still remains a major threat to public health. Among the various pathogens causing food borne outbreaks, Salmonella sps, Stapylococcus aureus, Bacillus cereus, Campylobacter sps have been incriminated for majority of outbreak cases. Now – a – days consumption of minimally processed ready to eat (RTE) food products increases the risk of food borne diseases. The aim of the present investigation was to detect the presence of FSSAI notified six food pathogens from ready to eat food products. A total of 250 samples (including milk, milk products, meat and meat products) were screened for the presence of food-borne bacterial pathogens like Staphylococcus aureus, Bacillus cereus, Campylobacter jejuni, Streptococcus sp and Salmonella sp on selective medium. Further, culture positive colonies were screened by colony morphology, gram staining, biochemical analysis and molecular methods. Antibiotic susceptibility test was performed for all isolates of Staphylococcus aureus, Bacillus cereus and Streptococcus spp. The results revealed that the Staphylococcus aureus had 100% resistant against Vancomycin, Methicillin and Penicillin antibiotics and Bacillus cereus isolates had 100% resistant against Vancomycin and Penicillin antibiotics and 100% sensitive towards Methicillin. Whereas the Streptococcus sp. shows 100% resistant against Gentamicin, Streptomycin, Tetracycline and Ciprofloxacin. The prevalence of multidrug resistant pathogenic isolates in ready to eat food products may act as potential source of foodborne intoxication under favourable conditions which need to be addressed.


Keywords: Food-borne pathogens, ready to eat (RTE) food products, PCR detection
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How to cite this article:

Rathnapraba. S, B. J. Shathish Sharma, P. Kanagaraju, S. Meignanalakshmi and Manoharan. S. 2020. Detection and Molecular Characterization of Food Borne Pathogens from Ready to Eat Meat and Milk Products.Int.J.Curr.Microbiol.App.Sci. 9(5): 944-951. doi: https://doi.org/10.20546/ijcmas.2020.905.104