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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 891-906
DOI: https://doi.org/10.20546/ijcmas.2020.905.098


Cytotoxicity Effects of Lactobacillus acidophilus on Hep G2 Cell Line and its Effects on Gene Regulation with Bax and Bcl2
N. G. Ramesh Babu1, K. A. Simrah Fathima2*, V. Nandhini3 and V. Nandhini4
Department of Biotechnology, Adhiyamaan College of Engineering, Hosur, Tamilnadu, India
*Corresponding author
Abstract:

The microorganisms most commonly used as probiotics are lactic acid bacteria, especially those of the genus Lactobacillus. In the present study, Lactobacillus acidophilus were isolated from two different pooled samples homemade curd and commercial curd, were characterized for their antiproliferative activity. The antiproliferative effects of the strains were investigated using the MTT assay with liver cancer (Hep G2) cell line. The results showed that the Lactobacillus strains had good antiproliferative effects in liver cancer cell line. Further the viability of cells was observed with the help of fluorescent microscopy by dual staining technique. Lactobacillus acidophilus can be considered as potential probiotic bacteria for humans because of their antiproliferation effect in cancer cells. In this study, effect of the samples on expression of Bax and Bcl2 gene was analysed by RT -PCR. β-Actin was chosen as an internal control to normalize the gene expression. The study indicate that the MTT assay for the strains of Lactobacilus acidophilus isolated from the sample has cytotoxicity effect. Based on the IC50 value, homemade curd showed better percent of inhibition than commercial curd and Bcl2 gene showed fold up regulation when compared to Bax gene.


Keywords: Probiotic; Lactobacillus; Antiproliferation; Hep G2; Bax and Bcl2 gene
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How to cite this article:

Ramesh Babu. N. G., K. A. Simrah Fathima, V. Nandhini and Nandhini. V. 2020. Cytotoxicity Effects of Lactobacillus Acidophilus on Hep G2 Cell Line and its Effects on Gene Regulation with Bax & Bcl2.Int.J.Curr.Microbiol.App.Sci. 9(5): 891-906. doi: https://doi.org/10.20546/ijcmas.2020.905.098