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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 743-750
DOI: https://doi.org/10.20546/ijcmas.2020.905.082


Comparative Efficacy of Diagnostic Methodology for Detection of Canine Parvo Virus Faecal Antigen
S. A. Mehta1*, S. V. Mavadiya1, A. A. Vagh2, S. M. Parmar1, J. A. Vala1 and R. M. Patel3
1Veterinary College, Navsari Agricultural University, Navsari -396450 (Gujarat), India
2Veterinary College, JAU, Junagadh, India
3Veterinary College, SDAU, Sardarkrushinagar, India
*Corresponding author
Abstract:

The study was conducted to compare diagnostic efficacy of two techniques viz. Scan Vet Parvo rapid faecal antigen detection kit and haemagglutination (HA) test with polymerized chain reaction (PCR). A total of 145 fecal samples from dogs showing similar clinical signs of parvovirus infection were collected aseptically from Veterinary Clinical Complex, Veterinary College, Navsari. The ScanVet Parvo was able to detect CPV antigen in 34 (68.00%) samples out of 50. The HA test found positive in 44(30.34%) samples, While using PCR, 63 samples (86.00%) were found positive out of 145 including 9 and 19 negative samples of ScanVet Parvo and HA test, respectively. However, both the test showed 100% specificity and sensitivity 79.07% (Scan Vet Parvo) and 69.84 % (HA) versus PCR. Thus, it is suggested that ScanVet Parvo could be employed for the preliminary screening in field condition and HA test in the laboratory. However, negative samples should further be confirmed through PCR.


Keywords: Canine Parvovirus, ScanVet Parvo HA, Dog, PCR, Fecal samples
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How to cite this article:

Mehta. S. A., S. V. Mavadiya, A. A. Vagh, S. M. Parmar, J. A. Vala and Patel. R. M. 2020. Comparative Efficacy of Diagnostic Methodology for Detection of Canine Parvo Virus Faecal Antigen.Int.J.Curr.Microbiol.App.Sci. 9(5): 743-750. doi: https://doi.org/10.20546/ijcmas.2020.905.082