National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An investigation was undertaken on fractionation of secondary metabolites from Ocimum sanctum and Aloe barbadensis Mill to check their antifungal activity against Aspergillus niger and Sclerotium rolfsii. Isolation of secondary metabolites was done by cold percolation and soxhlet extraction method with Methanol. Cold percolation method was found effective to get more phytochemicals. Analysis was done to check the presence of phytochemicals likes Alkaloids, Tannins, Saponins, Phenols, Flavonoids, Glycosides, Steroids and Terpenoids. Also qualitative tests viz. detection of Alkaloids was carried out by Wagner’s reagent, Glycosides by Keller-killiani test, steroids by concentrated H2SO4, Tannins by 0.1N HCl, Saponins by distilled water, Phenol by Ferric chloride, Flavonoids by concentrated H2SO4. Except phenol all components were present in both Ocimum sanctum and Aloe barbadensis Mill leaves extract. Presence of these components was determined by colour change or foam development after adding reagents into extract. Thin layer chromatography was done for the separation of different chemical constituents present in methanolic extracts. During standardization of eight different solvent systems Chloroform: Ethyl acetate : Acetic acid (50:50:1 v/v) found effective for the separation of Ocimum sanctum and Toluene : Ethyl acetate (93:7 v/v) for Aloe barbadensis Mill for the seperation of different components. scrapping of desired bands on TLC of partially purified compound used to check the antifungal activity against Aspergillus niger and Sclerotium rolfsii using poisoned food method. Different concentrations i.e. 20 mg, 40 mg, and 60 mg were taken, amongst 60 mg showed significant growth inhibition as against 20 mg.
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